Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach

Journal of Immunological Methods
F WulfertM J C Alcocer

Abstract

The sera of a retrospective cohort (n=41) composed of children with well characterized cow's milk allergy collected from multiple visits were analyzed using a protein microarray system measuring four classes of immunoglobulins. The frequency of the visits, age and gender distribution reflected real situation faced by the clinicians at a pediatric reference center for food allergy in São Paulo, Brazil. The profiling array results have shown that total IgG and IgA share similar specificity whilst IgM and in particular IgE are distantly related. The correlation of specificity of IgE and IgA is variable amongst the patients and this relationship cannot be used to predict atopy or the onset of tolerance to milk. The array profiling technique has corroborated the clinical selection criteria for this cohort albeit it clearly suggested that 4 out of the 41 patients might have allergies other than milk origin. There was also a good correlation between the array data and ImmunoCAP results, casein in particular. By using qualitative and quantitative multivariate analysis routines it was possible to produce validated statistical models to predict with reasonable accuracy the onset of tolerance to milk proteins. If expanded to larger study ...Continue Reading

References

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Jun 16, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M C García-AraM Martín-Esteban
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Oct 27, 2010·Journal of Immunological Methods·N K RenaultM J C Alcocer

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Citations

Oct 31, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K M Järvinen, J Celestin
Jul 22, 2018·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Hossein EsmaeilzadehFereshteh Mosavat

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