Predictive value of cervical cytokine, antimicrobial and microflora levels for pre-term birth in high-risk women

Scientific Reports
Rashmi ManningGendie E Lash

Abstract

Spontaneous preterm birth (sPTB, delivery <37 weeks gestation), accounts for approximately 10% of births worldwide; the aetiology is multifactorial with intra-amniotic infection being one contributing factor. This study aimed to determine whether asymptomatic women with a history of sPTB or cervical surgery have altered levels of inflammatory/antimicrobial mediators and/or microflora within cervical fluid at 22-24 weeks gestation. External cervical fluid was collected from women with history of previous sPTB and/or cervical surgery at 22-24 weeks gestation (n = 135). Cytokine and antimicrobial peptides were measured on a multiplex platform or by ELISA. qPCR was performed for detection of 7 potentially pathogenic bacterial species. IL-8 and IL-1β levels were lower in women who delivered preterm compared to those who delivered at term (IL-8 P = 0.02; IL-1β P = 0.04). There were no differences in elafin or human beta defensin-1 protein levels between the two groups. Multiple bacterial species were detected in a higher proportion of women who delivered preterm than in those who delivered at term (P = 0.005). Cervical fluid IL-8 and IL-1β and microflora have the potential to be used as biomarkers to predict sPTB in high risk women.

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Citations

Apr 28, 2021·American Journal of Reproductive Immunology : AJRI·Sunwha ParkYoung Ju Kim
Dec 3, 2021·PloS One·Nicholas R SpencerRamkumar Menon

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Methods Mentioned

BETA
Protein Assay
PCR

Clinical Trials Mentioned

ISRCTN14568373

Software Mentioned

Prism

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