Predictive value of mixed antigen screen beads in pre-transplant assessment of HLA immunization in solid organ transplant recipients.

Clinical Transplantation
Renaud SnanoudjJean-Luc Taupin

Abstract

Pre-transplant serum screening of anti-HLA antibodies is recommended for solid organ transplantations. Many laboratories use the less expensive bead-based screening assay as the main technique and, if positive, turn to single-antigen beads (SAB). We studied the correlations between these two immunoassays. We re-analyzed the raw data of the two assays in 3030 first organ transplant recipients, explored with the two tests. We performed a ROC curve analysis of the screening ratio to predict a positive SAB assay. The AUC were 0.72 and 0.64 for class I and class II. The optimal thresholds of screening ratios were 3.28 (class I) and 2.11 (class II). Whatever the class, the negative predictive value was low, around 40%, with 36% of discordant sera, as defined by negative screening and positive SAB. Testing class I discordant sera on acid-treated SAB showed that 54% of antibodies reacted against denatured HLA molecules. However, these screening-negative sera may contain donor-specific antibodies in 13.9% and 28.7% of cases for class I and class II, respectively, involved in antibody-mediated rejection with the same frequency as non-discordant sera. Given the low predictive value of screening, both assays should be performed at least on...Continue Reading

References

Aug 13, 2005·Bioinformatics·Tobias SingThomas Lengauer
Oct 24, 2008·Transplantation·Luis E Morales-BuenrostroJosefina Alberú
Jul 17, 2010·Journal of the American Society of Nephrology : JASN·Carmen LefaucheurCaroline Suberbielle-Boissel
Oct 5, 2010·Current Opinion in Organ Transplantation·Ilias I N DoxiadisFrans H J Claas
Nov 20, 2012·Journal of the American Society of Nephrology : JASN·Sumit MohanJai Radhakrishnan
Jan 12, 2013·Transfusion·Manish J GandhiUNKNOWN National Heart, Lung, and Blood Institute Specialized Center of Clinically Oriented Research TRALI Study and Retrovirus Epid
Jun 15, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·E F ReedD Gjertson
Jul 12, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P GombosC Süsal
Nov 15, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A SicardR Snanoudj
May 28, 2014·Transfusion·Dorien De ClippelPhilippe Vandekerckhove
Apr 1, 2015·European Journal of Immunology·Jonathan VisentinJean-Luc Taupin
Apr 30, 2015·British Journal of Haematology·Anna L PetersAlexander P J Vlaar
Aug 19, 2016·HLA : Immune Response Genetics·E G KamburovaH G Otten
Dec 20, 2017·Medical Sciences : Open Access Journal·UNKNOWN Organizing Committee CMHC 2017
Apr 1, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anat R TamburPeter Nickerson

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Citations

Aug 12, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Andrew M CourtwrightHilary J Goldberg

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