Prednisolone and prednisone neo-formation in bovine urine after sampling

Animal : an International Journal of Animal Bioscience
Francesco ArioliGiuseppe Pompa

Abstract

The rise in the frequency of detecting prednisolone in bovine urine from northern Italy has come into focus of attention in recent years. The possibility that neo-formation of prednisolone or that prednisone may occur in urine after collection of samples was therefore investigated. Cow urine collected for official routine controls in Lombardy containing more than 80 ng/ml cortisol, and prednisolone and prednisone below the decision limit (CCα) of the method (0.4 and 0.5 ng/ml, respectively) was used. The C1-2 dehydrogenation of naturally present cortisol and cortisone was checked by incubating urine, both contaminated and uncontaminated with faeces, at 37°C and by collecting samples at 0, 1, 2, 4, 6 and 24 h. The influence of Helix pomatia juice was also investigated in order to determine whether deconjugation could influence the reliability of the results. All samples were analysed by HPLC-MS3 for the presence of cortisol, cortisone, prednisolone and prednisone in negative electrospray ionisation mode, utilising the consecutive reaction monitoring of product ions derived from the formate molecular adduct ([M+HCOO]-). The observed neo-formation of prednisolone shows that inappropriate temperatures in sample storage and processi...Continue Reading

References

Dec 19, 2007·Rapid Communications in Mass Spectrometry : RCM·Francesco ArioliGiuseppe Pompa
Feb 27, 2008·Analytica Chimica Acta·H NoppeH F De Brabander
Jun 13, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Karolien VerheydenHubert F De Brabander
Apr 28, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Nick J KoszewskiHartmut H Malluche
Jan 5, 2011·Drug Testing and Analysis·Thomas PiperWilhelm Schänzer
Mar 25, 2011·The Journal of Endocrinology·Yewei XingWilliam E Rainey

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Citations

Jan 15, 2014·Journal of the Science of Food and Agriculture·Luca ChiesaFrancesco Arioli
Apr 16, 2016·Journal of Animal Physiology and Animal Nutrition·L ChiesaE Manuali
Nov 28, 2012·Steroids·Marco FidaniFrancesco Arioli
Jun 17, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Luca ChiesaFrancesco Arioli
Nov 9, 2019·Drug Testing and Analysis·Monica MazzarinoFrancesco Botrè
Oct 28, 2019·Drug Testing and Analysis·Loredana IannellaXavier de la Torre

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