Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae

Biology Open
Lei HuoYaozeng Xu

Abstract

Patients taking glucocorticoid or glucocorticoid-like drugs for an extended period of time may develop osteoporosis, termed glucocorticoid-induced osteoporosis (GIOP). GIOP is the most common form of secondary osteoporosis, but the mechanism underlying its development is unclear. In the present study, we used prednisolone to treat zebrafish larvae to investigate GIOP. Our RNA deep-sequencing (RNA-seq) results show that prednisolone affects genes known to act in the extracellular region. Therefore the extracellular region, extracellular matrix, and collagen trimer might be involved in glucocorticoid-induced osteoporosis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the focal adhesion signaling pathway is the most enriched signaling pathway in terms of differentially expressed genes (DEGs). In this pathway, integrin subunit alpha 10 (itga10) and integrin subunit beta like 1 (itgbl1), genes encoding two adapter proteins, were down-regulated in the prednisolone-treated larvae. Further experiments showed that prednisolone contributes to GIOP by down-regulating itga10 and itgbl1.

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Citations

Aug 28, 2020·Frontiers in Endocrinology·Francesca TonelliAntonella Forlino
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Yufei XieMarcel J M Schaaf
Jan 24, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kristin DietrichFranziska Knopf
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Joana T RosaM Leonor Cancela

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Methods Mentioned

BETA
whole
RNA-seq
PCR
RNA Assay
Assay

Software Mentioned

Office
DEGseq R package ( Biomarker
cBot Cluster Generation System
SPSS
Excel
edgeR

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