Prednisolone inhibits hyperosmolarity-induced expression of MCP-1 via NF-kappaB in peritoneal mesothelial cells

Kidney International
H MatsuoY Nakashima

Abstract

The mechanism of peritoneal fibrosis in patients on continuous ambulatory peritoneal dialysis (CAPD) is poorly elucidated. We investigated the cellular mechanism of high-glucose-induced expression of monocyte chemoattractant protein-1 (MCP-1), which is important in recruiting monocytes into the peritoneum and progression of peritoneal fibrosis, and examined the inhibitory mechanism of glucocorticoids. Rat peritoneal mesothelial cells were cultured in high-glucose-containing medium and then analyzed for phosphorylation levels of p42/44 and p38 mitogen-activated protein (MAP) kinases (MAPK), MAPK or extracellular signal-regulated kinase kinase (MEK)1/2, c-Jun N-terminal kinase (JNK)1/2, and protein kinase C (PKC) by Western blotting. Expression of MCP-1 was examined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. DNA-binding activity of nuclear factor (NF)-kappaB was measured by electrophoretic mobility shift assay. High glucose increased MCP-1 mRNA and MCP-1 protein expression. Although glucose increased phosphorylation of MEK1/2, p42/44 MAPK, p38 MAPK, JNK1/2, and PKC, and DNA-binding activity of NF-kappaB, its effect on MCP-1 expression was suppressed only by PKC and NF-k...Continue Reading

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Citations

May 8, 2010·International Urology and Nephrology·Tom Cornelis, Dimitrios G Oreopoulos
Jul 11, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kuan-Yu HungTun-Jun Tsai
Nov 21, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Tetsu MiyamotoYutaka Otsuji
Mar 4, 2011·American Journal of Physiology. Renal Physiology·Montserrat M Diaz EncarnacionJoseph P Grande
Jul 2, 2011·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Satoshi KokuboTakashi Wada
May 24, 2012·Mediators of Inflammation·Christoph KüperWolfgang Neuhofer
Oct 27, 2015·BioMed Research International·Harald SeegerStephan Segerer
Dec 19, 2015·Journal of the American Society of Nephrology : JASN·Li WangShougang Zhuang
Apr 3, 2015·American Journal of Physiology. Renal Physiology·Daniel KittererNiko Braun
May 8, 2010·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Ryouji OgataMasanori Kitamura
Apr 7, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryoji KojimaMikio Ito
Feb 14, 2021·In Vitro Cellular & Developmental Biology. Animal·Sepideh Alinezhad-BermiHana Hanaee-Ahvaz

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