Predominance of HCV type 2a in saliva from intravenous drug users

Journal of Medical Virology
K M RoyA Pithie

Abstract

Paired serum and saliva samples were collected simultaneously from 50 intravenous drug users with serologically proven hepatitis C virus infection. The oral health of the volunteers was also assessed. Hepatitis C virus RNA was detected by nested PCR, employing primers from the 5' noncoding region. Positive PCR products were sequenced using the Sequenase PCR Product Sequencing Kit (Amersham Life Sciences). HCV RNA was detected in 33 (66%) of the 50 serum samples. HCV RNA was detected in 19 (57.6%) of the corresponding 33 saliva samples. There was no correlation between oral health status or HIV seropositivity and the detection of HCV in saliva. However, subjects with HCV in their saliva were significantly more likely to complain of xerostomia (P < 0.05). Isolate genotypes were identified in paired serum and saliva of 15 intravenous drug users. HCV genotypes 1, 2, 3 and 6 were detected in both specimens. In seven cases, a differing HCV genotype was found in serum compared to the paired saliva specimen. The distributions of genotypes in serum and saliva were very different, with genotype 2a more common in saliva than serum (P < 0.005). These data suggest that in some cases the source of salivary HCV may not be serum transudation a...Continue Reading

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Citations

May 11, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Soraya de Mattos Camargo GrossmannMaria Auxiliadora Vieira do Carmo
Mar 18, 2011·Journal of Medical Virology·Luciane Almeida AmadoAna Maria Coimbra Gaspar
Feb 8, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sheila O Cameron, William F Carman
Jun 6, 2015·Journal of Infection and Public Health·Renata L Xavier SantosJurema F L de Castro
Mar 19, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M Castro FerreiroP Diz Dios
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M Carrozzo, S Gandolfo
Aug 2, 2012·Memórias do Instituto Oswaldo Cruz·Gisele Barreto Lopes MenezesMitermayer G Reis
Dec 29, 2005·AIDS Research and Human Retroviruses·M EireaA Ocampo
May 22, 2001·Journal of Medical Virology·O SaridM Margalith
Aug 27, 2005·Journal of Medical Virology·Liliane LinsMitermayer G Reis
Nov 24, 1999·Oral Diseases·K Roy, J Bagg

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