Predominant D1 Receptors Involvement in the Over-expression of CART Peptides after Repeated Cocaine Administration

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Zhenzhen HuKi-Wan Oh

Abstract

The aim of this study was to investigate the involvement of dopaminergic receptors (DR) in behavioral sensitization, as measured by locomotor activity, and the over-expression of cocaine- and amphetamine-regulated transcript (CART) peptides after repeated administration of cocaine in mice. Repeated administrations of cocaine induced behavioral sensitization and CART over-expression in mice. The levels of striatal CART mRNA were significantly increased on the 3(rd) day. CART peptides were over-expressed on the 5(th) day in the striata of behaviorally sensitized mice. A higher proportion of CART(+) cells in the cocaine-treated mice were present in the nucleus accumbens (NAc) shell than in the dorsolateral (DL) part of caudate putamen (CP). The concomitant administration of both D1R and D2R antagonists, SCH 23390 (D1R selective) and raclopride (D2R selective), blocked cocaine induced-behavioral sensitization, CART over-expression, and cyclic adenosine 5'-monophosphate (cAMP)/protein kinase A (PKA)/phospho-cAMP response element-binding protein (pCREB) signal pathways. SCH 23390 more predominantly inhibited the locomotor activity, CART over-expression, pCREB and PKA activity than raclopride. Cocaine induced-behavioral sensitization ...Continue Reading

Citations

Jan 1, 2016·Journal of Drug and Alcohol Research·Michael J Kuhar
Jul 24, 2018·Frontiers in Synaptic Neuroscience·Annette Gower, Mario Tiberi

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Methods Mentioned

BETA
PCR
Assay
genotyping
light microscopy
enzyme-linked immunoassay
immunoprecipitation

Software Mentioned

Fusion CAPI

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