Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor.

BMC Biology
Miryana MirchevaHans-Georg Koch

Abstract

The signal recognition particle (SRP) receptor plays a vital role in co-translational protein targeting, because it connects the soluble SRP-ribosome-nascent chain complex (SRP-RNCs) to the membrane bound Sec translocon. The eukaryotic SRP receptor (SR) is a heterodimeric protein complex, consisting of two unrelated GTPases. The SRbeta subunit is an integral membrane protein, which tethers the SRP-interacting SRalpha subunit permanently to the endoplasmic reticulum membrane. The prokaryotic SR lacks the SRbeta subunit and consists of only the SRalpha homologue FtsY. Strikingly, although FtsY requires membrane contact for functionality, cell fractionation studies have localized FtsY predominantly to the cytosolic fraction of Escherichia coli. So far, the exact function of the soluble SR in E. coli is unknown, but it has been suggested that, in contrast to eukaryotes, the prokaryotic SR might bind SRP-RNCs already in the cytosol and only then initiates membrane targeting. In the current study we have determined the contribution of soluble FtsY to co-translational targeting in vitro and have re-analysed the localization of FtsY in vivo by fluorescence microscopy. Our data show that FtsY can bind to SRP-ribosome nascent chains (RNC...Continue Reading

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Citations

May 6, 2011·The Journal of Biological Chemistry·Goran StjepanovicIrmgard Sinning
Apr 13, 2010·Journal of Preventive Medicine and Public Health = Yebang Ŭihakhoe Chi·Mina SuhChang Soo Kim
Feb 19, 2013·Annual Review of Biochemistry·David AkopianShu-ou Shan
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Apr 26, 2014·Molecular Membrane Biology·Kärt DenksHans-Georg Koch
Aug 17, 2010·European Journal of Cell Biology·Christine V RichterDanja Schünemann
Oct 16, 2015·The Journal of Cell Biology·Patrick KuhnHans-Georg Koch
Aug 5, 2010·Biochimica Et Biophysica Acta·Eitan Bibi
Feb 12, 2014·Biochimica Et Biophysica Acta·Ishu Saraogi, Shu-ou Shan
Jun 30, 2016·Molecular Microbiology·Albena DraychevaWolfgang Wintermeyer
Nov 13, 2012·Nature Structural & Molecular Biology·Wolf HoltkampWolfgang Wintermeyer
May 24, 2018·FEMS Microbiology Letters·Ruth SteinbergHans-Georg Koch
Feb 6, 2015·Journal of Cell Science·Adi Bercovich-Kinori, Eitan Bibi
May 4, 2021·Frontiers in Molecular Biosciences·Julia OswaldHans-Georg Koch
May 18, 2021·Frontiers in Microbiology·Benjamin MayerPeter L Graumann
Mar 10, 2017·EcoSal Plus·Andreas KuhnRoss E Dalbey
Mar 30, 2021·The FEBS Journal·Ruth Steinberg, Hans-Georg Koch

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Methods Mentioned

BETA
GTPase
fluorescence microscopy
electrophoresis
PCR

Software Mentioned

Autodeblur
Imagequant
Metamorph
Autodeblur X

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