Predominant recognition of species-specific determinants of the GroES homologues from Mycobacterium leprae and M. tuberculosis

Immunology
B Chua-IntraH M Vordermeier

Abstract

The Mycobacterium leprae and M. tuberculosis 10,000 MW heat-shock protein homologues of GroES have previously been identified as major immunogens for human T cells. We used synthetic peptides to characterize the determinants recognized by murine T cells. The findings suggest that, despite 90% sequence identity between these two proteins, T cells recognize prominently the species-specific determinants localized within amino acid residues 21-40 and 49-72. Analysis of the molecular determinants of species-specificity for the M. leprae GroES sequence 25-40, using T-cell hybridomas and major histocompatibility complex (MHC)-binding assays, led to the identification of epitope cores and critical residues. Interestingly, closely overlapping epitope cores were found to be restricted by either H-2Ad (24-34) or H-2Ed (28-34). Furthermore, the site recognized by the M. leprae-specific monoclonal antibodies ML06 and ML10 was also localized in the overlapping sequences 25-31 and 25-29. In conclusion, we demonstrated that immunodominant species-specific T- and B-cell epitopes can be found in a mycobacterial heat-shock protein despite its highly conserved amino acid sequence. This finding suggests the feasibility of identifying a sufficient n...Continue Reading

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Citations

Jul 7, 1999·Veterinary Microbiology·A J Cobb, R Frothingham
Dec 22, 2005·Infection and Immunity·Romulo AráozStewart T Cole
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Guglielmo LuccheseDarja Kanduc
Aug 9, 2006·Molecular Immunology·Stephanie CarmicleSamuel J Landry
Apr 10, 2014·Frontiers in Immunology·Juraj Ivanyi
Oct 24, 2001·The Journal of Biological Chemistry·Stephanie CarmicleSamuel J Landry

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