Preenrichment Conditions for Effective Recovery of Salmonella in Foods and Feed Ingredients

Journal of Food Protection
J Y D'Aoust, C Maishment

Abstract

The efficacy of Clausen, EE, Eugon, GN, Tergitol 7, lactose and nutrient broths as Salmonella preenrichment media was evaluated using 165 food samples with an incident contamination level ranging from 1.5 to 460 salmonellae/100 g. Replicate food samples (100 g) were preenriched in each of seven media (900 ml) for 6 h and 24 h at 35 C; various amounts (10, 1.0 and 0.1 ml) of preenriched cultures were selectively enriched in tetrathionate brilliant green (43 C) and selenite cystine (35 C) broths and plated on bismuth sulfite and brilliant green sulfa agars. Short (6 h) and 24-h preenrichment conditions resulted in 26 (16%) and 8 (5%) false negative results, respectively. Recovery of Salmonella from 6-h but not 24-h preenrichment cultures also varied directly with the portion of culture inoculated into selective enrichment broths. None of the preenrichment media tested performed satisfactorily at 6 h of incubation where levels of recovery ranged from 32 to 62%; at 24 h, good recovery was obtained with all media (95 to 100%) except EE broth (74%). The incidence of competitive flora was significantly higher on selenite + brilliant green sulfa than on tetrathionate + bismuth sulfite; transfer volumes (10 and 1.0 ml) and preenrichment...Continue Reading

Citations

Oct 1, 1984·The Journal of Applied Bacteriology·B M Mackey, C M Derrick
Sep 1, 1985·The Journal of Applied Bacteriology·M van Schothorst, A M Renaud
Aug 1, 1987·The Journal of Applied Bacteriology·C R Fricker
Sep 1, 1992·Journal of Dairy Science·F E el-Gazzar, E H Marth
Oct 1, 1981·Applied and Environmental Microbiology·T E Surdy, S O Haas
Feb 1, 1982·Applied and Environmental Microbiology·P EntisG A Jarvis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.