Preferential accumulation of GABAA receptor gamma 2L, not gamma 2S, cytoplasmic loops at rat spinal cord inhibitory synapses

The Journal of Physiology
J Meier, R Grantyn

Abstract

Alternative splicing generates two variants of the GABAAR gamma2-subunit, gamma2S and gamma2L, which differ by insertion of the amino acid sequence LLRMFSFK into the large cytoplasmic loop between transmembrane domains 3 and 4. This additional sequence within the GABAAR gamma2L-subunit contains the potential protein kinase C (PKC) phosphorylation site serine 343 (Ser343). In the present study we intended to determine the capacity of these two splice variants to accumulate at inhibitory synaptic terminals and to colocalize with gephyrin, and to find out whether phosphorylation of Ser343 has any effect on GABAAR distribution. Green fluorescent protein (GFP)-tagged large cytoplasmic loops of GABAAR gamma2S and gamma2L (GFP::gamma2S/L) were used as surrogates for full-length receptors to study the function of the individual gamma2S and gamma2L peptides in transfected spinal cord neurones (SCNs) and COS-7 cells. It was found that GFP::gamma2L displayed a significantly higher capacity to accumulate at inhibitory synapses than GFP::gamma2S. GABAAR GFP::gamma2S accumulation at inhibitory postsynaptic sites was suppressed to the extent that GFP::gamma2S assumed a diffuse cytosolic distribution. PKC activation facilitated the postsynapti...Continue Reading

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Citations

Jan 28, 2005·The European Journal of Neuroscience·Christian HennebergerRosemarie Grantyn
Apr 15, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kentaro MizutaCharles W Emala
Jul 29, 2011·Journal of Neurophysiology·Wanhua ShenHollis T Cline
Apr 17, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wanhua ShenHollis T Cline
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Jul 14, 2010·The Journal of Biological Chemistry·Leila SaiepourKirsten Harvey
Sep 8, 2018·Frontiers in Cellular Neuroscience·Joshua M Lorenz-GuertinTija C Jacob
Mar 27, 2021·Frontiers in Neurology·Neal H Barmack, Vito Enrico Pettorossi
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Sep 30, 2010·Neuroscience·N H BarmackV Yakhnitsa

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