Preferential adhesion of leukocytes near bifurcations is endothelium independent.

Microvascular Research
Nazanin TousiBalabhaskar Prabhakarpandian

Abstract

Leukocyte-endothelial interactions play central roles in many pathological conditions. However, the in vivo mechanisms responsible for nonuniform spatial distribution of adhering leukocytes to endothelial cells in microvascular networks are not clear. We used a combination of in vitro and in vivo methodologies to explain of this complex phenomenon. A mouse cremaster muscle model was used to study the spatial distribution of leukocyte-endothelial cell interaction in vivo. A PDMS-based synthetic microvascular network (SMN) device was used to study interactions of functionalized microspheres using a receptor-ligand system in a (endothelial) cell-free environment for the in vitro studies. Our in vivo and in vitro findings indicate that both leukocytes in vivo and microspheres in vitro preferentially adhere near bifurcation (within 1-2 diameters from the bifurcation). This adhesion pattern was found to be independent of the diameter of the vessels. These findings support our hypothesis that the fluidic patterns near bifurcations/junctions, and not the presence or cellular aspects of the system (e.g. cell deformation, cell signaling, heterogeneous distribution of adhesion molecules), is the main controlling factor behind the preferen...Continue Reading

Citations

Jan 15, 2014·Annals of Biomedical Engineering·Dmitry A FedosovSubra Suresh
Apr 5, 2013·Microfluidics and Nanofluidics·Jifu TanYaling Liu
Dec 23, 2011·Biomaterials·Kwang Hoon SongJunsang Doh
Dec 24, 2015·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Gerhard Gompper, Dmitry A Fedosov
Mar 23, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Balabhaskar PrabhakarpandianKapil Pant
Jul 19, 2011·Microvascular Research·Balabhaskar PrabhakarpandianMohammad F Kiani
Jun 4, 2015·Biophysical Journal·Etienne LoiseauManouk Abkarian
Feb 25, 2015·Microvascular Research·Giuseppina LambertiKapil Pant
Jun 16, 2017·Lab on a Chip·Boyang Zhang, Milica Radisic
Nov 6, 2018·Nanotechnology·Dawn S Y LinBoyang Zhang
Jun 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fariborz SoroushMohammad F Kiani
Mar 29, 2019·International Journal of Molecular Sciences·Qingliang YangLaurie E Kilpatrick
Jun 6, 2017·Cellular and Molecular Bioengineering·Jevgenia Zilberman-RudenkoJeevan Maddala
May 30, 2014·Experimental Biology and Medicine·Michael W IrvinAmbra Pozzi
May 1, 2021·Cancers·Yang ZhaoJennifer Gamble
Aug 8, 2021·International Journal of Molecular Sciences·Qingliang YangLaurie E Kilpatrick
Aug 20, 2014·Analytical Chemistry·Giuseppina LambertiMohammad F Kiani

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