Preferential and rapid degradation of raw rice starch by an α-amylase of glycoside hydrolase subfamily GH13_37

Applied Microbiology and Biotechnology
Yin LeiYi Gao

Abstract

The α-amylase (AmyP) from a marine metagenomic library belongs to the recently classified glycoside hydrolase subfamily GH13_37. The degradation abilities of AmyP on a broad range of raw starch granules were examined at 40 °C and pH 7.5. It was found that AmyP is a raw starch-degrading enzyme, exhibiting a unique and remarkable ability to preferentially and very rapidly digest raw rice starch. The specific activity of raw rice starch was reached 118.5 ± 0.6 Umg(-1), which was much higher than that of other raw starches. The final hydrolysis degrees were obtained in 4 h for 1 % raw rice starch and 1 h for 8 % concentration, indicating a very rapid speed of hydrolysis. The presence of a starch residue resistant was the main limiting factor for complete hydrolysis, although end product inhibition also existed, especially at high starch concentrations. AmyP randomly attacks unique or susceptible sites on raw rice starch granules, and releases glucose, maltose, and maltotriose as end products. This is the first biochemical characterization of the raw starch-degrading ability of an α-amylase of family GH13_37. The specific ability towards raw rice starch has never been described before, and this makes AmyP a promising candidate for u...Continue Reading

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Citations

Apr 2, 2013·Applied and Environmental Microbiology·Mónica Martínez-MartínezManuel Ferrer
Jun 29, 2013·Cellular and Molecular Life Sciences : CMLS·Štefan JanečekE Ann MacGregor
Nov 8, 2015·Applied Microbiology and Biotechnology·Marinela Šokarda SlavićNataša Božić
May 8, 2016·Cellular and Molecular Life Sciences : CMLS·Štefan Janeček, Marek Gabriško
Apr 1, 2018·Protein Science : a Publication of the Protein Society·Gregory ArnalNicole M Koropatkin
Aug 11, 2017·Biomolecular NMR Assignments·Xinxin LiXuecheng Zhang
Sep 20, 2019·Biotechnology Advances·Štefan JanečekBirte Svensson
Mar 15, 2021·Applied Biochemistry and Biotechnology·Jai Shankar PaulShailesh Kumar Jadhav
Jul 20, 2021·Gut Microbes·Peter A Dobranowski, Alain Stintzi

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