Preferential Budding of Vesicular Stomatitis Virus from the Basolateral Surface of Polarized Epithelial Cells Is Not Solely Directed by Matrix Protein or Glycoprotein

Journal of Virology
Eugene DrokhlyanskyConstance L Cepko

Abstract

Vesicular stomatitis virus has been shown to bud basolaterally, and the matrix protein, but not glycoprotein, was proposed to mediate this asymmetry. Using polarized T84 monolayers, we demonstrate that no single viral protein is sufficient for polarized budding. Particles are released from the apical and basolateral surfaces and are indistinguishable, indicating that there is no apical assembly defect. We propose that aspects of host cell polarity create a more efficient budding process at the basolateral surface.

References

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Citations

Dec 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Eugene DrokhlyanskyConstance L Cepko

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Methods Mentioned

BETA
confocal microscopy
transfection
transmission electron microscopy
glycosylation
glycosylations

Software Mentioned

ImageJ
ImageQuant

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