Preferential cleavage of chromatin-bound cohesin after targeted phosphorylation by Polo-like kinase

The EMBO Journal
Nadine C D Hornig, F Uhlmann

Abstract

The final irreversible step in the duplication and dissemination of eukaryotic genomes takes place when sister chromatid pairs split and separate in anaphase. This is triggered by the protease separase that cleaves the Scc1 subunit of 'cohesin', the protein complex responsible for holding sister chromatids together in metaphase. Only part of cellular cohesin is bound to chromosomes in metaphase, and it is unclear whether and how separase specifically targets this fraction for cleavage. We established an assay to compare cleavage of chromatin-bound versus soluble budding yeast cohesin. Scc1 in chromosomal cohesin is significantly preferred by separase over Scc1 in soluble cohesin. The difference is most likely due to preferential phosphorylation of chromatin-bound Scc1 by Polo-like kinase. Site-directed mutagenesis of 10 Polo phosphorylation sites in Scc1 slowed cleavage of chromatin-bound cohesin, and hyperphosphorylation of soluble Scc1 by Polo overexpression accelerated its cleavage to levels of chromosomal cohesin. Polo is bound to chromosomes independently of cohesin's presence, providing a possible explanation for chromosome-specific cohesin modification and targeting of separase cleavage.

References

May 1, 1994·The Journal of Cell Biology·V GuacciD Koshland
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M Yanagida
Dec 5, 2000·Current Biology : CB·W D WarrenC E Sunkel
Apr 4, 2001·The Journal of Cell Biology·P P BuddeR Heald
May 24, 2001·Cell·P V JallepalliC Lengauer
Dec 19, 2001·Cell·O StemmannM W Kirschner
Mar 22, 2002·Nature Reviews. Cancer·P V Jallepalli, C Lengauer
Apr 5, 2002·Molecular Cell·Izabela SumaraJan-Michael Peters
Jul 19, 2002·Current Biology : CB·Nadine C D HornigFrank Uhlmann
Feb 8, 2003·Current Biology : CB·Frank Uhlmann
Feb 25, 2003·Nature Cell Biology·Matt Sullivan, Frank Uhlmann
Nov 15, 2003·Current Biology : CB·Stefan WeitzerFrank Uhlmann
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christian H Haering, Kim Nasmyth

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Citations

Feb 11, 2005·Chromosoma·Olaf StemmannIngo H Gorr
Jul 17, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michele M P LufinoRichard Wade-Martins
Apr 13, 2010·Nature Cell Biology·Tadashi IshiguroYoshinori Watanabe
Jul 31, 2010·Nature Reviews. Drug Discovery·Klaus Strebhardt
Mar 24, 2009·Nature Reviews. Molecular Cell Biology·Vincent Archambault, David M Glover
Nov 3, 2010·The Journal of Biological Chemistry·Ying-Chou Chen, Michael Weinreich
Oct 17, 2008·Genes & Development·Carlos Egydio de CarvalhoMonica P Colaiácovo
Apr 27, 2011·Molecular and Cellular Biology·W-S HsuD J Clarke
Mar 25, 2010·Annual Review of Biochemistry·Bo Xiong, Jennifer L Gerton
Jul 12, 2008·Annual Review of Cell and Developmental Biology·Itay OnnDouglas E Koshland
Jul 25, 2009·Journal of Cell Science·Nobuaki R KudoKim Nasmyth
Oct 10, 2006·Nature Chemical Biology·Campbell McInnesDavid Glover
Feb 24, 2016·Biochimica Et Biophysica Acta·Shiv KumarJaebong Kim
Apr 19, 2011·Seminars in Cell & Developmental Biology·Hui Zou
May 14, 2008·Biochimica Et Biophysica Acta·Ana Losada
Oct 2, 2009·Clinical Genetics·J Liu, I D Krantz
Jun 9, 2012·Biochimica Et Biophysica Acta·Gunjan D MehtaSantanu Kumar Ghosh
May 14, 2010·The Biochemical Journal·Katherine M FeeneyJoanna L Parish
Mar 17, 2011·The Biochemical Journal·Hoi Tang Ma, Randy Y C Poon
Sep 22, 2009·Current Opinion in Cell Biology·Peter De WulfRosella Visintin
Jun 29, 2010·Cell Cycle·Cornelia RumpfJuraj Gregan
Feb 18, 2011·Nucleus·Martin Kucej, Hui Zou
Nov 21, 2007·Chemistry & Biology·Jennifer L SneadKevan M Shokat
Feb 4, 2014·Current Biology : CB·Lisa M D'Ambrosio, Brigitte D Lavoie
Sep 18, 2009·Developmental Cell·Meng-Fu Bryan TsouPrasad V Jallepalli
Jul 11, 2008·Molecular Cell·Jill M Heidinger-PauliDouglas Koshland
May 27, 2016·Molecular Biology of the Cell·Prashant K MishraMunira A Basrai
Aug 28, 2016·Trends in Cell Biology·Julia Kamenz, Silke Hauf
Mar 31, 2015·PLoS Genetics·Krystina L HoVivien Measday
Feb 24, 2017·Molecular Biology of the Cell·Vladimir V BotchkarevJames E Haber
Apr 7, 2017·Nature Structural & Molecular Biology·Martin R Singleton, Frank Uhlmann
Apr 20, 2005·Oncogene·Marcel A T M van Vugt, René H Medema
Jan 11, 2005·Oncogene·Junjun Liu, James L Maller
Mar 17, 2006·Nature·Tomoya S KitajimaYoshinori Watanabe

❮ Previous
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