Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2

Circulation Research
T SuzukiR Nagai

Abstract

Investigation of the molecular mechanisms that control smooth muscle cell (SMC) development and differentiation is a prerequisite in understanding the regulatory mechanisms of physiological and pathological SMC-associated vascular processes. The pluripotent murine embryonal carcinoma P19 cell, whose developmental potential resembles that of early embryonic cells, can develop into cell types derived from the neuroectoderm, mesoderm, and endoderm. In the present study, we have shown a unique strategy to enhance SMC differentiation in P19 cells. Under chemical induction of high concentrations of retinoic acid (1 micromol/L), P19 cells showed optimum differentiation into SMCs. Because the P19 cells thus induced also showed differentiation into neuronal cells, a strategy to block neuronal lineage differentiation was developed using a stable transformant antisense RNA construct against Brn-2, a neuronal lineage-specific POU-domain transcription factor; thus, by specifically inhibiting neuronal differentiation, enhanced SMC differentiation by P19 cells was attained. SMC expression was confirmed by immunohistochemical staining, RNA analysis (RNase protection assay), and protein analysis (Western blot) using SMC-specific markers (eg, SM...Continue Reading

References

Mar 1, 1992·The American Journal of Physiology·P BabijM Periasamy
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y HaraM Nirenberg
Nov 12, 1990·FEBS Letters·M GimonaJ V Small
Jan 1, 1990·Journal of Cellular Physiology·M A RudnickiM W McBurney
Nov 11, 1988·Nucleic Acids Research·B H MinD N Foster
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R NagaiM Periasamy
Dec 1, 1983·Molecular and Cellular Biology·M K EdwardsM W McBurney
Aug 1, 1982·The Journal of Cell Biology·E M Jones-VilleneuveV I Kalnins
Sep 6, 1993·FEBS Letters·P StrasserJ V Small

❮ Previous
Next ❯

Citations

Nov 19, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Akira UrunoKazuhisa Takeuchi
Aug 23, 2011·Journal of Biomedical Materials Research. Part a·Karen K NgMyron Spector
Feb 24, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D P AguiarJ G Abreu
Aug 17, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K BerggrenC J Forehand
Oct 21, 2006·Cells, Tissues, Organs·Wen-Chi C LeeKacey G Marra
Jun 15, 2000·Nature Cell Biology·I MoranoM Bader
Mar 26, 2002·Canadian Journal of Physiology and Pharmacology·G L WrightB S Chertow
Sep 23, 1997·Biochemical and Biophysical Research Communications·K OhmiY Nonomura
Mar 17, 1997·Biochemical and Biophysical Research Communications·K HasegawaY Matsuda
Apr 21, 1998·Bioconjugate Chemistry·I HabusS Agrawal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.