Preferential homing of passively transferred T cells into skin allografts of mice

Transplantation
A E Chang, P H Sugarbaker

Abstract

An assay which uses two differentially labeled cell populations was used to characterize the preferential localization of passively transferred syngeneic cells immunized to specific alloantigens. Splenocytes cytotoxic to B10.D2 and B10.BR alloantigens were harvested from (C57BL/6 X A/J)F1 (B6AF1) donors bearing acutely rejected skin allografts. One population was labeled in vitro with 3H-thymidine and the other with 14C-thymidine. The labeled cells were pooled and then transferred i.v. into B6AF1 hosts bearing 5-day-old skin grafts from B10.D2 and B10.BR donors. After 48 hr the mice were killed, and the relative amount of cells present in the skin grafts and draining axillary lymph nodes was derived by comparing the 3H:14C ratios of the harvested tissues. The results of these studies indicated that cytotoxic splenocytes harvested from donors bearing acutely rejected skin allografts preferentially localize to the relevant skin allograft after passive systemic transfer. The homing behavior of these splenocytes was augmented by T cell enrichment and significantly diminished by pretreatment with anti-Thy-1.2 serum plus rabbit complement. There was no evidence of preferential homing within the draining axillary lymph nodes. It can b...Continue Reading

Citations

Sep 29, 1999·Transplantation Proceedings·C LoinazE Moreno
May 1, 1981·The Journal of Experimental Medicine·B E LovelandI F McKenzie
Oct 1, 1982·The Journal of Experimental Medicine·A NemlanderP Häyry
Feb 1, 1982·Cellular Immunology·M M Kemeny, P H Sugarbaker
Jun 1, 1982·The Journal of Surgical Research·M M Kemeny, P H Sugarbaker
Apr 1, 1989·Immunological Reviews·J W Kupiec-Weglinski, N L Tilney
Jan 1, 1984·Immunological Reviews·N L AscherR L Simmons
Nov 1, 1991·Arthritis and Rheumatism·M Ziff

Related Concepts

Metazoa
Lymphocyte Immune Globulin, Anti-Thymocyte Globulin (Equine)
Cell Motility
Immunologic Tumoricidal Activities
Graft Vs Host Reaction
Normal Serum Globulin Therapy
Mice, Inbred A
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Inbred DBA

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