Preferential Interaction between the dopamine D2 receptor and Arrestin2 in neostriatal neurons

Molecular Pharmacology
Tara A MaceyKim A Neve

Abstract

Dopamine D2 receptor interactions with arrestins and arrestin-dependent internalization have been characterized using heterologously expressed D2 receptor and arrestins. The purpose of this study was to investigate D2 receptor interaction with endogenous arrestins. Arrestin2 and arrestin3 in striatal homogenates bound to the third cytoplasmic loop of the D2 receptor, and purified arrestin2 and arrestin3 bound to the second and third loops and C terminus of the D2 receptor, in a glutathione S-transferase pull-down assay. In NS20Y neuroblastoma cells expressing an enhanced green-fluorescent protein-tagged D2 receptor (D2-EGFP), 2-h D2 agonist stimulation enhanced the colocalization of D2-EGFP with endogenous arrestin2 and arrestin3. These results suggest that the D2 receptor has the intrinsic ability to bind both nonvisual arrestins. Agonist treatment of D2-EGFP NS20Y cells induced D2 receptor internalization (36-46%) that was maximal within 20 min, but that was prevented by small interfering RNA-induced depletion of arrestin2 and arrestin3. In neostriatal neurons, 2-h agonist treatment selectively increased the colocalization of the endogenous D2 receptor with arrestin2, whereas receptor colocalization with arrestin3 was reduced...Continue Reading

References

May 23, 1998·Annual Review of Pharmacology and Toxicology·J G Krupnick, J L Benovic
May 24, 2000·The Journal of Biological Chemistry·T A BennettE R Prossnitz
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L M LuttrellR J Lefkowitz
Sep 3, 2002·The Journal of Biological Chemistry·Jessica L DeGraffJeffrey L Benovic
Mar 6, 2004·The Journal of Biological Chemistry·Giovanni TulipanoStefan Schulz
Apr 23, 2004·Trends in Pharmacological Sciences·Vsevolod V Gurevich, Eugenia V Gurevich

❮ Previous
Next ❯

Citations

Jun 27, 2007·Journal of Molecular Histology·Elizabeth C WolstencroftGlenn E Morris
Jun 20, 2013·Behavioural Brain Research·Sira Díaz-MoránAlberto Fernández-Teruel
Apr 24, 2010·Expert Reviews in Molecular Medicine·Vsevolod V Gurevich, Eugenia V Gurevich
Dec 4, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ningning GuoStephen Rayport
Jul 13, 2012·The Journal of Biological Chemistry·Luis E GimenezVsevolod V Gurevich
May 2, 2006·The Journal of Biological Chemistry·Michael R BruchasCharles Chavkin
Dec 2, 2011·Journal of Neurophysiology·Arash Hadipour-NiktarashThomas Wichmann
Feb 19, 2010·Cellular and Molecular Life Sciences : CMLS·Pieter RondouKathleen Van Craenenbroeck
Dec 20, 2015·Journal of Neurochemistry·Nathalie SchmiegClotilde Mannoury la Cour
Oct 21, 2015·Expert Opinion on Drug Discovery·Wilhelmus E A de WitteElizabeth C M de Lange
Mar 17, 2007·Journal of Receptor and Signal Transduction Research·Yong LiuKim A Neve
Dec 7, 2013·Journal of Molecular Signaling·Pratima PandeyHarbinder S Dhillon
Jan 28, 2012·Journal of Molecular Signaling·Pratima Pandey, Singh Harbinder
Mar 19, 2005·Journal of Neurochemistry·Tara A MaceyKim A Neve
Mar 25, 2009·Synapse·Mette SkinbjergDavid R Sibley
Mar 6, 2010·Annals of the New York Academy of Sciences·Bryan K YamamotoGary A Gudelsky
Feb 8, 2006·Pharmacology & Therapeutics·Vsevolod V Gurevich, Eugenia V Gurevich
Aug 27, 2014·Biological Psychiatry·Christine DeLorenzoIrina Esterlis
Oct 29, 2013·Neuropharmacology·Angélica Osorio-EspinozaJosé-Antonio Arias-Montaño
Jun 21, 2006·Synapse·Philip SeemanTomiki Sumiyoshi
Sep 20, 2006·The Journal of Biological Chemistry·Tara A MaceyCharles Chavkin
Apr 12, 2019·Biomolecules & Therapeutics·Han-Sol YangKa Young Chung
Feb 23, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seol-Ae LeeSang Ki Park
Nov 21, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sophie E HolmesIrina Esterlis
May 9, 2006·Experimental Neurology·Luisa IacovelliFrancesco Fornai
Nov 26, 2009·Cellular Signalling·Anneleen SpoorenKathleen Van Craenenbroeck
Jul 30, 2005·Cell·James A Bibb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.