Preferential localization of the endocytic internalization machinery to hyphal tips underlies polarization of the actin cytoskeleton in Aspergillus nidulans

Molecular Microbiology
Lidia Araujo-BazánEduardo A Espeso

Abstract

AbpA, SlaB and AmpA, three demonstrated components of the endocytic internalization machinery, are strongly polarized in Aspergillus nidulans hyphae, forming a ring that embraces the hyphal tip, leaving an area of exclusion at the apex. AbpA, a prototypic endocytic internalization marker, localizes to highly motile and transient (average half life, 24 +/- 5 s) peripheral punctate structures overlapping with actin patches, which also predominate in the tip. SlaB also localizes to peripheral patches, but these are markedly more abundant and cortical than those of AbpA. In contrast to its polarized distribution in hyphae, endocytic patches show random distribution during the isotropic growth phase preceding polarity establishment, but polarize as soon as a germtube primordium emerges from the swelled conidiospore. Thus, while endocytosis can occur along the hyphae, the apical predominance and the spatial organization of actin patches and of the above endocytic machinery proteins as a slightly subapical ring strongly suggests that tight spatial coupling of apical secretion and subapical compensatory endocytosis underlies hyphal growth. In agreement, the phenotype of a null slaB allele indicates that endocytosis is essential.

References

Dec 1, 1988·The Journal of Cell Biology·D G DrubinD Botstein
Jan 1, 1986·Journal of Cell Science. Supplement·J MarksJ S Hyams
Dec 1, 1983·Gene·J TilburnR W Davies
Jun 11, 1998·The Journal of Biological Chemistry·R A Yamashita, G S May
Nov 5, 1998·Molecular Biology of the Cell·X S YeS A Osmani
Jan 19, 2000·Molecular Biology of the Cell·M J LewisH R Pelham
May 2, 2001·The Journal of Cell Biology·B L GoodeD G Drubin
Jun 18, 2002·The EMBO Journal·Roland Wedlich-SöldnerGero Steinberg
Nov 30, 2002·Current Opinion in Microbiology·Michelle Momany
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·Eckart D GundelfingerBritta Qualmann
Sep 19, 2003·Current Biology : CB·Javier Valdez-Taubas, Hugh R B Pelham
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Asa E Y Engqvist-Goldstein, David G Drubin
Oct 24, 2003·Genetics·Xiaorong Lin, Michelle Momany
Nov 26, 2003·Journal of Molecular Biology·Javier Fernández-MartínezEduardo A Espeso
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Jan 27, 2004·Molecular Biology of the Cell·Asa E Y Engqvist-GoldsteinDavid G Drubin
May 25, 2004·Molecular Biology of the Cell·Claire L PearsonSteven D Harris
Nov 19, 2004·Molecular Biology of the Cell·Tetsuya Horio, Berl R Oakley
Feb 11, 2005·Eukaryotic Cell·Steven D HarrisMichelle Momany
Sep 10, 2005·Eukaryotic Cell·Lois M DouglasJames B Konopka
Nov 18, 2005·Fungal Genetics and Biology : FG & B·Miguel A Peñalva
Feb 24, 2006·Nature·Tobias C WaltherPeter Walter

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Citations

May 21, 2011·Current Microbiology·Marta Cotado-SampayoFrançois Barja
Jan 22, 2013·Antonie van Leeuwenhoek·Jan Dijksterhuis, Douwe Molenaar
Dec 6, 2008·The Journal of Biological Chemistry·Olga Rodríguez-GalánMiguel A Peñalva
Jan 25, 2008·Molecular Biology of the Cell·Naimeh Taheri-TaleshBerl R Oakley
Nov 28, 2008·Molecular Biology of the Cell·Nadine Zekert, Reinhard Fischer
Aug 21, 2009·Molecular Biology of the Cell·Areti Pantazopoulou, Miguel A Peñalva
Mar 30, 2012·Molecular Biology of the Cell·Juan F AbenzaMiguel A Peñalva
Jul 19, 2013·Proceedings. Biological Sciences·Luke M Noble, Alex Andrianopoulos
Jun 10, 2008·Eukaryotic Cell·Soo Chan LeeBrian D Shaw
Aug 10, 2010·Eukaryotic Cell·Ioannis VangelatosVicky Sophianopoulou
Feb 9, 2010·Eukaryotic Cell·Adokiye BerepikiNick D Read
Jul 3, 2013·Annual Review of Microbiology·Meritxell Riquelme
Jul 5, 2013·PloS One·Ane Markina-IñarrairaeguiMiguel A Peñalva
Feb 18, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Zachary SchultzhausBrian D Shaw
Jan 26, 2016·Fungal Genetics and Biology : FG & B·Fernando Lara-RojasRosa R Mouriño-Pérez
Jun 29, 2011·The Journal of Cell Biology·Jun ZhangXin Xiang
Feb 12, 2013·Fungal Genetics and Biology : FG & B·Alexandros AthanasopoulosVicky Sophianopoulou
Jul 7, 2011·Seminars in Cell & Developmental Biology·Yuan Qin, Zhenbiao Yang
Jun 7, 2011·Fungal Biology·Brian D ShawSrijana Upadhyay
Jun 7, 2011·Fungal Biology·Hans-Peter Schmitz, Peter Philippsen
Jun 7, 2011·Fungal Biology·Steven D Harris
Feb 3, 2009·Current Opinion in Cell Biology·Brian J Galletta, John A Cooper
Aug 11, 2015·Molecular Microbiology·Erika Herrero-GarciaEduardo A Espeso
Sep 18, 2015·Molecular Biology of the Cell·Xuanli YaoXin Xiang
Jul 25, 2009·Molecular Microbiology·Steven D Harris

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