Preferential localization of the Epstein-Barr virus (EBV) oncoprotein LMP-1 to nuclei in human T cells: implications for its role in the development of EBV genome-positive T-cell lymphomas

Journal of Virology
Jingwu XuJosé Menezes

Abstract

The Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP-1) is thought to play a role in the EBV-induced B-cell transformation and immortalization. EBV has also been implicated in certain human T-cell lymphomas; however, the phenotypic effects of the expression of this oncoprotein in T cells are not known. To learn whether LMP-1 also induces phenotypic changes in T cells, we stably expressed it in human cell lines of T and B lineages and 25 LMP-1-expressing T-cell clones and 7 B-cell clones were examined. Our results show for the first time that, in sharp contrast to B cells, LMP-1 preferentially localizes to nuclei in T cells and does not induce the phenotypic changes in these cells that it induces in B cells, does not associate with TRAF proteins, and does not arrest the cell cycle in the G2/M phase. A computer-assisted analysis revealed that LMP-1 lacks the canonical nuclear localization signal. Our results suggest that this oncoprotein may not play the same role in the lymphomagenesis of T cells as it does in B cells.

References

Dec 1, 1992·British Journal of Haematology·H SuzushimaK Takatsuki
Apr 1, 1991·The Journal of Experimental Medicine·D WatryC D Tsoukas
Aug 1, 1988·European Journal of Immunology·C D Tsoukas, J D Lambris
Jul 1, 1987·Journal of Virology·K P Mann, D Thorley-Lawson
Jul 1, 1987·Molecular and Cellular Biology·D LiebowitzE Kieff
Sep 1, 1995·Microbiological Reviews·R KhannaD J Moss
Feb 1, 1993·Histopathology·G KhanG Slavin
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M KayeE Kieff
Mar 25, 1998·Current Biology : CB·A G Eliopoulos, A B Rickinson
Jun 26, 1998·Current Opinion in Cell Biology·L F PembertonJ S Rosenblum
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·W KulwichitN Raab-Traub
Oct 13, 1998·Immunity·G J BabcockD A Thorley-Lawson
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J XuJ Menezes

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Citations

Oct 3, 2014·PLoS Computational Biology·Andrew J FritzRonald Berezney
May 23, 2012·PloS One·David J WestonPaul S Freemont
Jun 28, 2005·Critical Reviews in Oncology/hematology·Anna L TaylorJ Andrew Bradley
Oct 19, 2004·Trends in Microbiology·Hans H NillerJanos Minarovits
Jul 2, 2003·Seminars in Arthritis and Rheumatism·Duane R Schultz, William J Harrington
Mar 22, 2018·Cancers·Kathy H Y ShairVaughn S Cooper
Jun 18, 2003·Journal of Cellular Physiology·Riccardo Dolcetti, Maria G Masucci
Dec 1, 2018·Future Virology·Mujeeb R Cheerathodi, David G Meckes

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