PMID: 9177906Mar 1, 1997Paper

Preferential localization of tyrosine-phosphorylated paxillin in focal adhesions

Cell Adhesion and Communication
A CattelinoI de Curtis

Abstract

Focal adhesions are sites for integrin-mediated attachment of cultured cells to the extracellular matrix. Localization studies have shown that focal adhesions can be stained by antiphosphotyrosine antibodies, but the role of tyrosine-phosphorylated proteins in focal adhesions is not known. By using ventral plasma membranes prepared from chicken embryo fibroblasts spread on the substrate, we present evidence for the preferential localization of a minor pool of tyrosine-phosphorylated paxillin in focal adhesions. Ventral plasma membranes showed an enrichment in beta 1-integrins, and in several tyrosine-phosphorylated polypeptides, while focal adhesion proteins like vinculin and paxillin, although localized to focal adhesions in ventral plasma membranes, were not particularly enriched in these preparations compared to whole cell lysates. Biochemical and morphological analysis of ventral plasma membranes showed a dramatic increase in the level of tyrosine-phosphorylation of the pool of paxillin localized to the adhesive sites, when compared to the paxillin present in whole cell lysates. The observed preferential localization of tyrosine-phosphorylated paxillin to focal adhesions may represent a general mechanism to compartmentalize...Continue Reading

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Citations

Jun 5, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ibrahima DiagneSheila G Haworth
Mar 21, 1998·Transplant Immunology·P F Davies
Feb 9, 1999·Molecular Biology of the Cell·A CattelinoI de Curtis
Mar 24, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jae-Won JungInn-Oc Han
Oct 7, 2004·Developmental Biology·Ming Li, Donald S Sakaguchi
Feb 24, 2001·Journal of Biomedical Materials Research·T G van KootenC J Kirkpatrick
May 14, 2005·Cancer Letters·Vered Givant-HorwitzReuven Reich
Jun 12, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ann M HopkinsAsma Nusrat

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