PMID: 9447235Feb 3, 1998Paper

Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts

Mutation Research
J J Salazar, Bennett Van Houten

Abstract

To test the hypothesis that mitochondrial DNA (mtDNA) is more prone to reactive oxygen species (ROS) damage than nuclear DNA, a continuous flux of hydrogen peroxide (H2O2) was produced with the glucose/glucose oxidase system. Using a horse radish peroxidase (HRPO)-based colorimetric assay to detect H2O2, glucose oxidase (GO; 12 mU/ml) produced 95 microM of H2O2 in 1 h, whereas only 46 microM of hydrogen peroxide accumulated in the presence of SV40-transformed human fibroblasts ( approximately 1 x 10(6). DNA damage was assessed in the mitochondira and three nuclear regions using a quantitative PCR assay. GO (12 mU/ml) resulted in more damage to the mitochondrial DNA (2.250 +/- 0.045 lesions/10 kb) than in any one of three nuclear targets, which included the non-expressed beta-globin locus (0.436 +/- 0.029 lesions/10 kb); and the active DNA polymerase b gene (0.442 +/- 0.037 lesions/10 kb); and the active hprt gene (0.310 +/- 0.025). Damage to the mtDNA occurred within 15 min of GO treatment, whereas nuclear damage did not appear until after 30 min, and reached a maximum after 60 min. Repair of mitochondrial damage after a 15 min GO (6 mU/ml) treatment was examined. Mitochondria repaired 50% of the damage after 1 h, and by 6 h al...Continue Reading

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W M BrownA C Wilson
Sep 1, 1992·Mutation Research·C Richter
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L G Baggetto
Nov 1, 1991·Kidney International·P D Walker, S V Shah
Sep 1, 1987·Biochemical Pharmacology·L O Lim, A H Neims
May 31, 1988·Biochemical and Biophysical Research Communications·A M Hruszkewycz
Jan 1, 1988·Progress in Nucleic Acid Research and Molecular Biology·J F Ward
Jan 1, 1988·Free Radical Biology & Medicine·A Bindoli
May 1, 1988·Mutation Research·R Menghini
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C RichterB N Ames
Oct 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·C Ody, A F Junod
Jul 1, 1995·Mutation Research·C C ShenG L Wilson
Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K ShigenagaB N Ames
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Agarwal, R S Sohal
Feb 1, 1994·Biochemical Society Transactions·M TreacyR M Hollingworth
Jul 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L Li, B H Lau
May 1, 1993·Biochemical Society Transactions·K J Davies
Feb 1, 1993·Neurology·H J Tritschler, R Medori
Apr 15, 1996·Nucleic Acids Research·P Jaruga, M Dizdaroglu
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·F M Yakes, B Van Houten

❮ Previous
Next ❯

Citations

Dec 18, 2001·Teratogenesis, Carcinogenesis, and Mutagenesis·E T Saliim, A Abu-Shakra
May 10, 2005·DNA Repair·Bennett Van HoutenJanine H Santos
Jun 1, 2013·DNA Repair·Inna N ShokolenkoMikhail F Alexeyev
Nov 13, 2002·Mutation Research·Bhaskar S MandavilliBennett Van Houten
Aug 29, 2003·Mutation Research·Dennis E SawyerR John Aitken
Mar 5, 1999·Mutation Research·K B Beckman, B N Ames
May 23, 2001·Toxicology Letters·S ZhouK B Wallace
Mar 23, 2002·Experimental Gerontology·Abdullah OlgunTurker Kutluay
Jul 13, 2000·Free Radical Biology & Medicine·S B HollensworthS P LeDoux
Apr 30, 2002·Free Radical Biology & Medicine·John W Eaton, Mingwei Qian
Oct 11, 2002·Free Radical Biology & Medicine·Felice D'Agnillo, Abdu I Alayash
Sep 16, 1999·Mutation Research·S P LeDouxG L Wilson
Sep 16, 1999·Mutation Research·D E Sawyer, B Van Houten
Sep 16, 1999·Mutation Research·L A Marcelino, W G Thilly
May 10, 2001·Mutation Research·J S PentaW C Copeland
Feb 9, 2006·Chemical Reviews·Maria A GraziewiczWilliam C Copeland
Apr 24, 2010·The Journal of Biological Chemistry·Jay M SageKendall L Knight
Feb 21, 2006·Antioxidants & Redox Signaling·Gustav Mattiasson, Patrick G Sullivan
Jun 19, 2007·Antioxidants & Redox Signaling·Masahiro OkouchiTak Yee Aw
Oct 7, 2009·Antioxidants & Redox Signaling·Montserrat MaríJosé C Fernández-Checa
Jun 28, 2002·Nucleic Acids Research·Maria A GraziewiczWilliam C Copeland
May 5, 2009·Cornea·Shari R AtilanoM Cristina Kenney
Feb 3, 1999·Annals of the New York Academy of Sciences·K B Beckman, B N Ames
Nov 25, 2003·American Journal of Respiratory Cell and Molecular Biology·Yaoyu NingLester Kobzik
Aug 24, 2013·Biological & Pharmaceutical Bulletin·Xingyu ZhaoZhengli Luo
Apr 7, 2000·Environmental Health Perspectives·B RihnG Keith
Mar 10, 2010·Biochemical and Biophysical Research Communications·Shengmin ZhouHirofumi Shoun
Aug 20, 2015·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sara C Zapico, Douglas H Ubelaker
Jun 21, 2005·Annals of the New York Academy of Sciences·Rong-Hong HsiehHsing-Hsien Cheng
Sep 24, 2004·Biochemical and Biophysical Research Communications·Jan A SikorskyJames Denvir
Apr 3, 2016·Free Radical Biology & Medicine·Yixing WuPeter L Oliver

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.