Preferential production of latent transforming growth factor beta-2 by primary prostatic epithelial cells and its activation by prostate-specific antigen

Journal of Cellular Physiology
S L DallasL F Bonewald

Abstract

Three mammalian isoforms of transforming growth factor-beta (TGFbeta) are known, TGFbeta1, 2, and 3, that have non-overlapping functions during development. However, their specific roles in cancers such as prostate cancer are less clear. Here we show that primary cultures of prostatic epithelial cells preferentially produce and activate the latent TGFbeta2 isoform. Paired cultures of normal and malignant prostate cells from prostate cancer patients produced predominantly the TGFbeta2 isoform, with 30- to 70-fold less TGFbeta1. By mono-Q ion exchange chromatography, three major peaks of latent TGFbeta2 activity were observed corresponding to the known small latent TGFbeta2 complex, the known large latent TGFbeta2 complex and a novel eluting peak of latent TGFbeta2. Although prostate cells are known to activate latent TGFbeta, the mechanism for activation is currently unclear. We investigated whether prostate specific antigen (PSA), a serine protease used as a clinical marker for prostate cancer, could play a role in the activation of latent TGFbeta. Unlike plasmin, a known activator of both latent TGFbeta1 and 2, PSA specifically activated the recombinant small latent form of TGFbeta2, but not TGFbeta1. Prostate epithelial cells...Continue Reading

References

Jan 1, 1990·Annals of the New York Academy of Sciences·D R TwardzikF W Ruscetti
Apr 28, 1989·Biochemical and Biophysical Research Communications·P Henttu, P Vihko
Dec 1, 1995·Nature Genetics·G ProetzelT Doetschman
Nov 1, 1994·Endocrinology·M S SteinerE R Barrack
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A B KulkarniS Karlsson
Dec 14, 1995·Biochimica Et Biophysica Acta·D DeperthesJ Y Dubé
Aug 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J TaipaleJ Keski-Oja
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J GerdesD R Rowley
Apr 3, 1998·Mineral and Electrolyte Metabolism·A B Roberts
Jul 11, 1998·The Journal of Biological Chemistry·J SaharinenJ Keski-Oja
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·S SinhaC M Kielty
Oct 8, 1999·Journal of the National Cancer Institute·A H FortierJ W Holaday
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S L DallasL F Bonewald
Feb 13, 2001·Journal of Cellular Physiology·B Pasche

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Citations

Aug 10, 2011·Prostate Cancer and Prostatic Diseases·A P CummingW C Duivenvoorden
Oct 27, 2007·Antioxidants & Redox Signaling·Katri KoliVuokko L Kinnula
Feb 15, 2014·Advances in Wound Care·Kenneth W FinnsonAnie Philip
May 24, 2008·Protein Engineering, Design & Selection : PEDS·Erik V VeronaLu-Zhe Sun
Jun 26, 2007·Cells, Tissues, Organs·Mitchell G LawrenceJudith A Clements
Jul 17, 2008·Biological Chemistry·Hannu KoistinenUlf-Håkan Stenman
May 17, 2014·Frontiers in Immunology·Harm WestdorpI Jolanda M de Vries
Mar 27, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nashmil Emami, Eleftherios P Diamandis
Apr 12, 2008·Molecular and Cellular Endocrinology·Paul A BerryAnne T Collins
Dec 20, 2015·PloS One·Jennifer S MyersQing-Xiang Amy Sang
Oct 24, 2007·Journal of Cellular Biochemistry·Jessica HaverkampTimothy L Ratliff
Apr 4, 2008·The Prostate·John T Isaacs
Jul 8, 2011·American Journal of Reproductive Immunology : AJRI·Heriberto Rodríguez-MartínezJuan J Calvete
Feb 23, 2020·Biomolecules·Alexander Batista-DuharteSalvador Francisco Aliño
Mar 12, 2011·The Prostate·Simon A WilliamsSamuel R Denmeade
Dec 23, 2014·Medicinal Research Reviews·Herve Aloysius, Longqin Hu
Jun 3, 2016·Cold Spring Harbor Perspectives in Biology·Ian B Robertson, Daniel B Rifkin
Mar 3, 2011·International Journal of Cancer. Journal International Du Cancer·Jung-Kang JinGary E Gallick
Jun 25, 2009·Scandinavian Journal of Clinical and Laboratory Investigation·Johanna M MattssonHannu Koistinen
Feb 13, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael L ManningSamuel R Denmeade
Sep 5, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Scott D CramerDonna M Peehl
Jan 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jairo OlivaresJohn Nemunaitis
Mar 15, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John M Chirgwin, Theresa A Guise
Jun 5, 2021·Journal of the American Chemical Society·Scott LovellEdward W Tate
Jul 29, 2020·Anti-cancer Agents in Medicinal Chemistry·Muhammad A B AshrafArif Malik
Oct 7, 2021·Nature Reviews. Cancer·Robert L Satcher, Xiang H-F Zhang

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