PMID: 8976185Dec 1, 1996Paper

Preferential proliferation of murine colony-forming units in culture in a chemically defined condition with a macrophage colony-stimulating factor-negative stromal cell clone

The Journal of Experimental Medicine
N TakakuraS Nishikawa

Abstract

The establishment of culture conditions that selectively support hematopoietic stem cells is an important goal of hematology. In this study, we investigated the possibility of using for this purpose a defined medium, mSFO2, which was developed for stromal cell-dependent bone marrow cultures. We found that a combination of epidermal growth factor (EGF), the OP9 stromal cell line, which lacks macrophage colony-stimulating factor, recombinant stem cell factor, and the chemically defined medium mSFO2 provides a microenvironment where c-Kit+ Thy-1+/lo Mac-1+/lo B220- TER119- common beta + IL-2R gamma + gp130+ cells are selectively propagated from normal, unfractionated bone marrow cells. This cell population produced an in vitro colony at a very high efficiency (50%), whereas it has only limited proliferative ability in the irradiated recipient. Thus, the cells selected in this culture condition might represent colony-forming units in culture (CFU-c) with short-term reconstituting ability. Transferring this cell population into medium containing differentiation signals resulted in the rapid production of mature myelomonocytic and B cell lineages in vitro and in vivo. The fact that a similar culture condition was created by erb-B2-tr...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M GormanA Miyajima
Jul 1, 1991·The Journal of Experimental Medicine·M OgawaS Nishikawa
Jan 1, 1991·Current Topics in Developmental Biology·S Heimfeld, I L Weissman
Jul 1, 1988·Science·G J SpangrudeI L Weissman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S GreenbergerR J Eckner
Jan 1, 1981·Molecular and Cellular Biology·E M ScolnickT M Dexter
Feb 11, 1982·Nature·M C MagliN Odartchenko
Jul 1, 1994·Leukemia & Lymphoma·M ShioharaA Komiyama
Jan 1, 1995·International Immunology·Y KimuraK Sugamura
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KalthoffW Schmiegel
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T SudoS Nishikawa

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Citations

Mar 24, 2004·Cytokine·Meng Ling ChoongHarvey F Lodish
Apr 12, 2006·The Journal of Experimental Medicine·Yoshihiro Yamada, Nobuyuki Takakura
Apr 14, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shanbao CaiKaren E Pollok
Nov 22, 2013·Circulation Journal : Official Journal of the Japanese Circulation Society·Nobuyuki Takakura
Aug 19, 2009·PloS One·Yui HaradaYoshikazu Yonemitsu
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Masanori SakaguchiHideyuki Okano
May 29, 2013·Genesis : the Journal of Genetics and Development·Ingo BurtscherHeiko Lickert
Mar 16, 2012·Cancer Science·Nobuyuki Takakura
Jul 2, 2003·Mechanisms of Development·Mitsunari NakajimaTakuji Shirasawa
Mar 12, 2013·The American Journal of Pathology·Takahiro MatsuiNobuyuki Takakura
Aug 26, 2003·FEBS Letters·Meng Ling ChoongHarvey F Lodish
Dec 2, 2004·Blood·Rika OkamotoNobuyuki Takakura

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