PMID: 2115571Aug 1, 1990Paper

Preferential rearrangement of V kappa 4 gene segments in pre-B cell lines

The Journal of Experimental Medicine
S L Kalled, P H Brodeur

Abstract

Examination of the in vitro V kappa gene rearrangements of murine adult bone marrow-derived pre-B cell lines reveals that 21 of 25 (84%) cell lines have rearranged a member of the V kappa 4 family. In contrast, analysis of two V kappa cDNA libraries prepared from LPS-stimulated adult spleen cells indicates that only 17% of the Ig kappa cDNAs contain sequences belonging to the V kappa 4 gene family. Half of the pre-B cell lines examined also share an 8-kbp BamHI reciprocal product (rp). However, these rp do not involve the same V kappa gene, indicating that conserved BamHI sites exist 3' of some V kappa genes. This rp is also readily detected in DNA from normal adult spleen cells, suggesting that the in vitro rearrangements examined in this study are representative of kappa rearrangements that occur in vivo. We suggest that, unlike the diverse V kappa repertoire expressed by mature B cells, the germline V kappa segments involved in initial rearrangements of the Ig kappa locus are highly restricted, and that an initial V kappa 4 rearrangement is probably followed by other, more random recombination events.

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Citations

Sep 21, 2004·Immunogenetics·Katherine M Brekke, William T Garrard
Aug 6, 2002·Seminars in Immunology·David NemazeeLaurent Verkoczy
Mar 1, 1995·The Journal of Experimental Medicine·J H RoarkJ Erikson
Jan 1, 1997·International Reviews of Immunology·A MeyerR A Goldsby
Jan 1, 1992·International Reviews of Immunology·J M Teale, C A Medina
Sep 20, 1991·Cell·A Rolink, F Melchers
Feb 1, 1995·European Journal of Immunology·A KaushikJ C Jaton
Apr 1, 1991·European Journal of Immunology·P SanchezP A Cazenave
Jun 15, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caroline GrönwallGregg J Silverman
Jan 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miyo Aoki-OtaDavid Nemazee

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