Preferential translation of reovirus mRNA by a sigma3-dependent mechanism

Virology
S SchmechelL Schiff

Abstract

We have characterized reovirus strains that differ in the degree to which they inhibit cellular protein synthesis and used them to investigate mechanisms regulating gene expression in infected cells. A previous genetic study associated distinct effects of reovirus strains on cellular translation with polymorphisms in viral protein sigma3. In cell extracts, sigma3 sequesters double-stranded RNA (dsRNA) and blocks activation of the dsRNA-activated protein kinase (PKR), an interferon-induced enzyme that inhibits translational initiation by phosphorylating elF-2alpha. We found that in infected cells, cellular protein synthesis is translationally regulated in a strain-specific manner. Using immunoprecipitation and indirect immunofluorescence we showed that the effect of a strain on cellular translation is not determined by the level of sigma3, but appears to result from differences in sigma3 localization. In cells infected with a strain that spares cellular translation, sigma3 is present throughout the cytoplasm, whereas in cells infected with inhibitory strains, sigma3 is restricted to perinuclear viral factories. Biochemical studies suggested that diffuse localization of sigma3 is a consequence of low affinity for capsid protein m...Continue Reading

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Citations

Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Jan 28, 2006·Journal of Virology·Jennifer A SmithLeslie A Schiff
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A Jelinsky, L D Samson
Oct 31, 2015·Virology·Irene Lostalé-SeijoJavier Benavente
Oct 20, 2006·Journal of Virological Methods·Michael R Roner, Christine Mutsoli
Jul 1, 2010·Wiley Interdisciplinary Reviews. RNA·Marta Maria Gaglia, Britt A Glaunsinger
Jan 18, 2015·Virus Research·Øystein WesselEspen Rimstad
Mar 29, 2018·Nature Microbiology·Cathy L Miller
Sep 18, 2014·MBio·Emily A DesmetJohn S L Parker
Mar 25, 2019·Viruses·Raquel TenorioTerence S Dermody
Feb 23, 2019·Journal of Virology·Anthony J Snyder, Pranav Danthi
Oct 22, 2003·Canadian Journal of Microbiology·Etienne Rouault, Guy Lemay
Jan 1, 2011·Future Virology·Cathy L Miller
Aug 28, 2020·Journal of Virology·Katherine E RoebkePranav Danthi

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