Preferential uptake of chitosan-coated PLGA nanoparticles by primary human antigen presenting cells

Nanomedicine : Nanotechnology, Biology, and Medicine
Verónica DuránClaus-Michael Lehr

Abstract

Biodegradable polymeric nanoparticles (NP) made from poly (lactid-co-glycolide) acid (PLGA) and chitosan (CS) hold promise as innovative formulations for targeted delivery. Since interactions of such NP with primary human immune cells have not been characterized, yet, here we assessed the effect of PLGA or CS-PLGA NP treatment on human peripheral blood mononuclear cells (PBMC), as well as on monocyte-derived DC (moDC). Amongst PBMC, antigen presenting cells (APC) showed higher uptake of both NP preparations than lymphocytes. Furthermore, moDC internalized CS-PLGA NP more efficiently than PLGA NP, presumably because of receptor-mediated endocytosis. Consequently, CS-PLGA NP were delivered mostly to endosomal compartments, whereas PLGA NP primarily ended up in lysosomes. Thus, CS-PLGA NP confer enhanced delivery to endosomal compartments of APC, offering new therapeutic options to either induce or modulate APC function and to inhibit pathogens that preferentially infect APC.

Citations

Nov 28, 2019·Influenza and Other Respiratory Viruses·Atin Khalaj-HedayatiKhai Wooi Lee
Jul 11, 2020·Biomimetics·Ana Maria Carmona-Ribeiro, Yunys Pérez-Betancourt
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Divesha EssaViness Pillay
Jul 3, 2020·Frontiers in Bioengineering and Biotechnology·Gopal PatelGuoyin Kai
Jun 12, 2020·Expert Opinion on Drug Delivery·Suchada NiamsuphapChristopher B Howard
Dec 31, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Gopal PatelUttam Chand Banerjee
Dec 29, 2020·Biomaterials·David WibowoBernd H A Rehm
Dec 22, 2020·Frontiers in Pharmacology·Concetta Di NataleDaniela Marasco
Apr 17, 2021·Journal of Nanobiotechnology·Humzah JamshaidGul Majid Khan

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