Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells

Veterinary Research
Jiexiong XieHans J Nauwynck

Abstract

Cellular entry mediators define whether the cell is permissive to PRRSV infection. Porcine sialoadhesin (pSn, Siglec-1) and CD163 are main entry mediators facilitating infection of porcine macrophages by PRRSV. Recently, Siglec-10 was demonstrated to be an alternative receptor for PRRSV. To examine if virulence and pathogenicity of PRRSV strains could be correlated with the use of different Siglecs, a PK15 cell line recombinantly expressing Siglec-1 and CD163 (PK15S1-CD163) and a PK15 cell line recombinantly expressing Siglec-10 and CD163 (PK15S10-CD163) were used to compare the virus replication of 7 genotype 1 subtype 1 strains (G1s1), 2 genotype 1 subtype 3 (G1s3) strains and 5 genotype 2 (G2) strains. Some strains (08VA (G1s1), 13V117 (G1s1), 17V035 (G1s1), VR2332 (G2)) were poor virus producers (<104 TCID50/mL), while other strains (07V063 (G1s1), 13V091 (G1s1), Su1-Bel (G1s3), MN-184 (G2), Korea17 (G2) and SDSU-73 (G2)) easily grew up to ≥106 TCID50/mL. PK15S10-CD163 cells exhibited a higher efficiency in virus production per infected cell than the PK15S1-CD163 cells. The G1s1 strains LV and 07V063 infected more cells in the PK15S1-CD163, whereas the 94V360 and 08VA strains preferred PK15S10-CD163. The highly virulent G1s...Continue Reading

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D A BenfieldD Chladek
Apr 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E M BautistaJ E Collins
May 29, 1998·The Journal of General Virology·E J Snijder, J J Meulenberg
Aug 27, 2005·Journal of the American Veterinary Medical Association·Eric J NeumannJeffrey J Zimmerman
May 12, 2007·Journal of Virology·Jay G CalvertSiao-Kun W Welch
Nov 15, 2008·The Journal of General Virology·Hanne Van GorpHans J Nauwynck
Apr 23, 2010·The Journal of General Virology·Wander Van BreedamHans J Nauwynck

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Citations

Jul 11, 2021·Veterinary Microbiology·I Ruedas-TorresJ Gómez-Laguna

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Methods Mentioned

BETA
FCS
Facs
flow cytometry
confocal
glycosylation

Software Mentioned

SPSS
Clustal Omega
Image J
GraphPad Prism

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