Prefrontal levels of 5-HIAA, but not dopamine, predict alcohol consumption in male Wistar rats following 6-OHDA lesions
Abstract
To examine the effects of dopamine (DA) on alcohol consumption, male Wistar rats were subjected either to 6-OHDA lesions of the frontal cortex (MPFC) or to a sham lesion/no lesion. Following surgery, rats were trained to drink alcohol on a sucrose-fading paradigm over the course of 6 weeks, at the completion of which they consumed a solution of 3% sucrose/10% alcohol. Daily consumption of alcohol was computed for each rat. Animals were sacrificed and the MPFC, nucleus accumbens (NA), and ventral tegmentum (VTA) were removed. Levels of DA and its metabolites (i.e., HVA and DOPAC), norepinephrine (NE), and serotonin (5-HT) and its metabolite (i.e., 5-HIAA) were measured for each brain region using HPLC with electrochemical detection. Post hoc analyses were run examining the relationship of DA and its metabolites, 5-HT and its metabolite (5-HIAA), and norepinephrine (NE) in the MPFC, NA, and VTA with alcohol consumption. The 6-OHDA lesions depleted DA to 74.5% of control levels in the MPFC, but did not significantly affect alcohol consumption. Post hoc analyses found that the "high" alcohol consumption group had significantly reduced levels of MPFC 5-HIAA in comparison to the "low" consumption group, but that there was no relation...Continue Reading
References
Microinjections of dopamine agonists in the nucleus accumbens increase ethanol-reinforced responding
Immunostained serotonergic fibers are decreased in selected brain regions of alcohol-preferring rats
Ethanol drinking following 6-OHDA lesions of nucleus accumbens and tuberculum olfactorium of the rat
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