Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation.

Scientific Reports
Eman Y Abu-RishYasser Bustanji

Abstract

Immune system alteration has been implicated in the pathogenesis of chronic pain conditions, epilepsy and generalized anxiety disorder. Targeting cytokines has recently been proposed for the management of such conditions. Pregabalin (PGB) is an antiepileptic agent used for the management of these conditions. However, little is known about its immunomodulatory effects on cytokine secretion in vivo and in vitro. Hence, a mitogen (Lipopolysaccharide [LPS] or Concanavalin A [ConA])-induced murine model of inflammation was used to investigate the effect of PGB on in vivo and in vitro IL-1β, IL-6, TNF-α and IL-2 cytokine secretion using ELISA. In addition, PGB effect on spleen histology, as a lymphoid organ, was examined. Our results revealed that PGB significantly inhibited the secretion of ConA-induced IL-6 secretion, basal and ConA-induced TNF-α and IL-2 secretion in splenocytes in vitro. In vivo, PGB inhibited basal and LPS/ConA-induced IL-6 and TNF-α secretion in addition to LPS-induced IL-1β and ConA-induced IL-2 secretion. Moreover, PGB attenuated mitogen-induced inflammatory changes in the spleen. These findings provide an evidence of the anti-inflammatory properties of PGB on cytokine secretion and lymphoid organ inflammatio...Continue Reading

References

Nov 1, 1994·Neuroimmunomodulation·G De SarroG Nisticó
Dec 18, 2001·Environmental Health Perspectives·H Tryphonas
Jul 27, 2005·Therapeutic Drug Monitoring·David Berry, Christopher Millington
Oct 28, 2006·Toxicologic Pathology·Mark F Cesta
Jan 10, 2008·International Immunopharmacology·Mohammad FararjehShtaywy Abdalla
Apr 20, 2010·Annals of the New York Academy of Sciences·C IannuccelliG Valesini
May 13, 2011·Epilepsia·Ettore Beghi, Simon Shorvon
Dec 7, 2011·Critical Reviews in Immunology·Manuela RossolSunna Hauschildt
Jun 26, 2012·Anesthesia and Analgesia·Yeon JangDae Chul Jeong
Aug 13, 2013·Mediators of Inflammation·Steven BiesmansRony Nuydens
Aug 28, 2013·Journal of Psychiatric Research·Hubertus HimmerichUlrich Sack
Dec 3, 2014·Neuropharmacology·Annamaria Vezzani, Barbara Viviani
May 3, 2015·Pharmacological Reports : PR·Barbara BłaszczykStanisław Jerzy Czuczwar
Jul 4, 2015·Journal of Leukocyte Biology·Emma KayJames R Whiteford
Aug 11, 2015·Frontiers in Immunology·Bennett DavenportAnne-Laure Perraud
Sep 12, 2015·Brain and Behavior·Baik-Lin EunSookyong Koh
Feb 16, 2016·Neuroscience and Biobehavioral Reviews·Evelien E de VriesFloor E Jansen
Feb 16, 2016·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Junad KhanVincent Ziccardi
Mar 27, 2016·Biochimica Et Biophysica Acta·Michelle RothaugStefan Rose-John
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nicholas H VarvelRaymond Dingledine
Dec 17, 2016·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Neetu Godhwani, Sami L Bahna
Dec 13, 2017·Scandinavian Journal of Pain·Alice L HungTina L Doshi
Dec 24, 2017·Journal of Neuroimmunology·Tiina AlapirttiJukka Peltola
Jan 18, 2018·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Eman Y Abu-RishYasser Bustanji
Feb 16, 2018·Pain·Claudia SommerNurcan Üçeyler
Jun 26, 2018·Journal of Neuroimmunology·Eman Y Abu-RishMichael J Browning
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rahime AslankocSanem Asci
Dec 6, 2018·The Eurasian Journal of Medicine·Fatma Sultan KilicSetenay Oner
Dec 26, 2018·Journal of Alzheimer's Disease : JAD·Shawn M BartonWellington Pham

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Citations

Aug 18, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Nora HegazyAhmed Fahmy
Mar 22, 2021·Toxicology and Applied Pharmacology·Flaviano Ribeiro Pinheiro-NetoFernanda Regina de Castro Almeida

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