PMID: 8583011Jul 1, 1995Paper

Pregnancies following pre-conception diagnosis of common aneuploidies by fluorescent in-situ hybridization

Human Reproduction
Y VerlinskyJ Moise

Abstract

Chromosomal aneuploidies contribute considerably to the low pregnancy rate in in-vitro fertilization (IVF). The objective of this experimental work was to explore the possibility of detecting common aneuploidies in oocytes by polar body sampling. The study included 45 infertile patients of advanced maternal age participating in an IVF programme. The first polar body was removed prior to fertilization or both the first and second polar bodies were removed after fertilization and studied by fluorescent in-situ hybridization (FISH) using chromosome-specific probes for chromosomes X, 18 and/or 13/21. Of 155 oocytes with FISH results, 36 demonstrated chromosomal abnormalities. Of 119 oocytes predicted to be free from aneuploidy of chromosomes X, 18 and/or 13/21, 72 were normally fertilized, cleaved and transferred in 23 treatment cycles, which resulted in two healthy deliveries and three ongoing pregnancies confirmed to be unaffected by chorionic villous sampling. The method may appear useful for the detection of oocytes with common chromosomal aneuploidies in IVF patients of advanced maternal age.

Citations

Feb 1, 1996·Journal of Assisted Reproduction and Genetics·Y Verlinsky
Feb 1, 1996·Journal of Assisted Reproduction and Genetics·J C Harper
Feb 1, 1996·Journal of Assisted Reproduction and Genetics·S RechitskyC M Strom
Feb 1, 1996·Journal of Assisted Reproduction and Genetics·Y VerlinskyA Kuliev
Jul 13, 2011·Human Genetics·Joyce C Harper, Sioban B Sengupta
Aug 1, 1997·Fertility and Sterility·A S Lim, M F Tsakok
Jan 1, 1999·Maturitas·U Eichenlaub-Ritter
Mar 30, 2006·Reproductive Biomedicine Online·Anver KulievYury Verlinsky
Apr 24, 2003·Reproductive Biomedicine Online·Esther VelillaSantiago Munné
Dec 25, 2009·Human Reproduction·Joep GeraedtsAndreas Schmutzler
Feb 1, 1997·Journal of Assisted Reproduction and Genetics·Y VerlinskyL Wilton
Dec 3, 2014·The Kaohsiung Journal of Medical Sciences·Levent SahinAyse Ender Yumru
Jul 16, 2014·Taiwanese Journal of Obstetrics & Gynecology·Chun-Kai ChenChyi-Long Lee
Jun 29, 2010·Fertility and Sterility·Joyce C Harper, Gary Harton
Jan 19, 2016·Reproductive Biomedicine Online·S B SenGuptaJ C Harper
Dec 24, 2010·Seminars in Fetal & Neonatal Medicine·E IwarssonE Blennow
Dec 1, 2010·Reproductive Biomedicine Online·Anver KulievJeanine Cieslak Janzen
Aug 20, 1996·Human Gene Therapy·J C Fletcher, G Richter
Sep 30, 2005·Cytogenetic and Genome Research·A KulievY Verlinsky
Aug 28, 2015·Human Fertility : Journal of the British Fertility Society·Virginia N BoltonJoyce C Harper
Sep 7, 2016·Reproductive Biology and Endocrinology : RB&E·Norbert GleicherUNKNOWN International PGS Consortium Study Group
Jun 16, 2017·Journal of Medical Screening·Joyce C Harper
May 29, 2002·Current Opinion in Obstetrics & Gynecology·Santiago Munné, Dagan Wells
Jul 17, 2009·Current Opinion in Obstetrics & Gynecology·Santiago MunnéDagan Wells
Apr 11, 2017·Systems Biology in Reproductive Medicine·Xavier VendrellMiguel Ruiz-Jorro
Jul 29, 2018·Journal of Assisted Reproduction and Genetics·Mara SimopoulouMichael Koutsilieris
Jul 19, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Romualdo SciorioJames Catt
Sep 9, 2020·The Cochrane Database of Systematic Reviews·Simone CornelisseSebastiaan Mastenbroek
Aug 21, 2004·Human Reproduction Update·Anver Kuliev, Yury Verlinsky
Jun 24, 2010·Prenatal Diagnosis·Joe Leigh Simpson
Jun 9, 2014·Science China. Life Sciences·LiYing YanJie Qiao
Apr 18, 2003·European Journal of Human Genetics : EJHG·Aïda PujolJoaquima Navarro
Feb 20, 2007·Annals of the New York Academy of Sciences·George B Maroulis, Nikoletta Koutlaki

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