Pregnancy and bovine somatotropin in nonlactating dairy cows: II. Endometrial gene expression related to maintenance of pregnancy

Journal of Dairy Science
A GuzelogluW W Thatcher

Abstract

The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bST-treated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor alpha (ERalpha) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERalpha protein, and bST stimulated an increase in ERalpha protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERalpha protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P ...Continue Reading

References

Aug 1, 1990·Biology of Reproduction·C E FarinR M Roberts
Apr 1, 1985·Biology of Reproduction·F F BartolW W Thatcher
Sep 1, 1982·Biology of Reproduction·G S LewisJ S Curl
Oct 22, 1994·The Veterinary Record·G E Mann, G E Lamming
Aug 1, 1994·Journal of Reproduction and Fertility·J H Payne, G E Lamming
Apr 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·C J NewtonG K Stalla
Dec 1, 1995·DNA and Cell Biology·R BathgateR Ivell
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa
Sep 24, 1999·Biology of Reproduction·H WangL Sahlin
Oct 3, 1999·Molecular Human Reproduction·L Marions, K G Danielsson
Dec 10, 1999·The Journal of Endocrinology·M FeldmanD L Kleinberg
Jul 6, 2000·Journal of Dairy Science·F MoreiraW W Thatcher
Feb 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·G E Mann, G E Lamming
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·R S RobinsonD C Wathes
May 30, 2002·The Journal of Biological Chemistry·Hiromichi MatsumotoSudhansu K Dey

❮ Previous
Next ❯

Citations

Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·H Erdem, A Guzeloglu
Nov 11, 2005·Journal of the American Veterinary Medical Association·Julian A BartolomeLouis F Archbald

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.