Pregnancy-associated corticosteroid-binding globulin: high resolution separation of glycan isoforms

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
E MitchellC J Bagley

Abstract

Corticosteroid-binding globulin (CBG) is a glycoprotein that functions as a specific carrier of cortisol in the circulation. CBG contains six sites for N-glycosylation with, on average, five sites occupied by a mixture of biantennary and triantennary oligosaccharides with variable additional terminal sialic acid residues leading to glycoforms with significant heterogeneity in mass and isoelectric points. During pregnancy, a form of CBG possessing only triantennary oligosaccharides comprising approximately 10 % of total CBG appears specifically. We describe the first application of two-dimensional gel electrophoresis to the separation of human CBG glycoforms. This technique resolved a greater degree of charge heterogeneity than previous studies, and allowed simultaneous visualization of changes to the size and isoelectric points of CBG during pregnancy. Profiles of CBG glycoforms during pregnancy showed a general increase in size followed by a shift to lower pI in a large proportion of the glycoprotein. This may result from the enhancement of triantennary glycosylation, with the extent of incorporation of sialic acid increasing with the number of available sites for its addition. The pregnancy-specific CBG previously defined pro...Continue Reading

Citations

May 12, 2011·Molecular & Cellular Proteomics : MCP·Zeynep Sumer-BayraktarMorten Thaysen-Andersen
Oct 24, 2006·Physiology & Behavior·Ari LevineAron Weller
Oct 13, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Robin MihrshahiSinan O Ali
Apr 12, 2016·Clinical Endocrinology·Marni A NenkeDavid J Torpy
Mar 14, 2017·Current Opinion in Endocrinology, Diabetes, and Obesity·Fabienne LangloisMaria Fleseriu
Aug 15, 2012·Nature Reviews. Endocrinology·Ilias PerogamvrosPeter J Trainer
Dec 24, 2017·Journal of Molecular Endocrinology·Marc SimardGeoffrey L Hammond
Jun 5, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Kristine Kollerøs PantonArne Åsberg
Jul 27, 2021·Journal of the Endocrine Society·Emily Jane MeyerDavid James Torpy
Mar 22, 2007·Journal of Proteome Research·Srinivasa R NagallaMary E D'Alton
Aug 1, 2009·Molecular and Cellular Endocrinology·Lucia GagliardiDavid J Torpy

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