Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy

Fertility and Sterility
Kiyonori MiuraHideaki Masuzaki

Abstract

To investigate cell-free pregnancy-associated microRNAs as molecular markers for the diagnosis of ectopic pregnancy. Laboratory study using human plasma samples. Research unit in a university hospital. Plasma samples from 18 women with ectopic pregnancies (EP group), 12 women with spontaneous abortion (SA group), and 26 normal women with singleton pregnancies (NP group). Total RNAs containing small RNA molecules extracted from 1.2 mL of plasma. Plasma concentrations of cell-free microRNAs measured by quantitative real-time reverse-transcriptase polymerase chain reaction. Plasma concentrations of cell-free pregnancy-associated microRNAs (miR-323-3p, miR-515-3p, miR-517a, miR-517c, and miR-518b) and serum concentration of human chorionic gonadotropin (hCG) were confirmed to have statistically significantly different plasma or serum concentrations in women with EP, SA, or NP. There was no statistically significant difference in the plasma concentrations of cell-free miR-21 between the three groups. By correlation coefficient analysis, no relationship was detected between serum hCG levels and plasma cell-free miR-517c, miR-515-3p, miR-517a, miR-518b, miR-323-3p, or miR-21 levels. Plasma concentrations of cell-free miR-517a could di...Continue Reading

References

Mar 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Enders K O NgY M Dennis Lo
Aug 16, 2005·Lancet·Cynthia M Farquhar
Mar 15, 2006·Cell·Ronald H A Plasterk
Jan 26, 2008·Clinical Chemistry·Stephen S C ChimY M Dennis Lo
Jun 3, 2008·Obstetrics and Gynecology·UNKNOWN American College of Obstetricians and Gynecologists
Jul 9, 2010·Human Molecular Genetics·Marie Noguer-DanceJérôme Cavaillé
Aug 24, 2010·Clinical Chemistry·Kiyonori MiuraHideaki Masuzaki
Sep 15, 2010·Clinical Chemistry·Rossa W K Chiu, Y M Dennis Lo
Mar 19, 2011·BMC Bioinformatics·Xavier RobinMarkus Müller
Mar 3, 2012·Molecular Human Reproduction·R B DonkerYoel Sadovsky
Jan 29, 2013·Prenatal Diagnosis·Ai HigashijimaHideaki Masuzaki
Feb 26, 2013·Journal of Reproductive Immunology·Diana M Morales-PrietoUdo R Markert
Jul 3, 2013·Clinical Chemistry·Yuri HasegawaHideaki Masuzaki
Feb 6, 2014·PloS One·Teresa LozoyaManuel Tena-Sempere
Jun 3, 2014·Fertility and Sterility·Daniela Galliano, Antonio Pellicer

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Citations

Sep 15, 2015·Frontiers in Neuroendocrinology·Bridget M Nugent, Tracy L Bale
Jan 1, 2016·Reproduction, Fertility, and Development·Dawit TesfayeKarl Schellander
Nov 8, 2018·BJOG : an International Journal of Obstetrics and Gynaecology·M MemtsaUNKNOWN Royal College of Obstetricians and Gynaecologists
May 14, 2019·Journal of Cellular Biochemistry·Xue HanLijie Yuan
Jul 7, 2020·BioMed Research International·Jianhua SunJie Gao
May 1, 2020·Medical Molecular Morphology·Shunsuke TamaruOsumu Ishihara
Oct 12, 2018·Molecular Cancer Therapeutics·Takeshi HisamatsuAnil K Sood
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hui GuZhengwei Yuan

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