Pregnancy associated plasma protein-A: interaction with heparin in crossed affinity immunoelectrophoresis

Australian and New Zealand Journal of Medicine
M SinosichJ G Grudzinskas

Abstract

A specific interaction between pregnancy associated plasma protein A- (PAPP A) and heparin has been demonstrated using heparin affinity crossed immunoelectrophoresis applied to late pregnancy serum. The presence of heparin in the first dimensional gel accelerated the anodic migration of six serum proteins with beta electrophoretic mobility and one protein in the alpha mobile region. Two of the six beta mobile proteins were identified as antithrombin III and beta lipoprotein, the alpha mobile protein being PAPP-A. The migration distance of other proteins originating in the placenta (pregnancy specific beta, glycoprotein), fetus (alpha-fetoprotein), leucocytes (pregnancy zone protein), or maternal liver (alpha, antitrypsin alpha 2 macroglobulin) was not altered. The interaction of PAPP-A with heparin was therefore independent of molecular size, charge, and site of origin indicating a specific high affinity interaction between PAPP-A and heparin. There results indicate that PAPP-A may be involved locally with the coagulation system in the maintenance of placental circulation.

Citations

Jan 1, 1983·Archives of Gynecology·J G WestergaardJ G Grudzinskas
Feb 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·B TeisnerJ G Grudzinskas
Mar 23, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Qiu-Ping QinKim Pettersson
Feb 1, 1983·British Journal of Obstetrics and Gynaecology·K M Toop, A Klopper
Dec 1, 1984·British Journal of Obstetrics and Gynaecology·J G WestergaardJ G Grudzinskas
Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·T Shimosegawa, S I Said
Apr 1, 1983·British Journal of Obstetrics and Gynaecology·A G Ahmed, A Klopper

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