Pregnancy increases ovine uterine artery endothelial cyclooxygenase-1 expression

Endocrinology
M A JanowiakI M Bird

Abstract

During normal pregnancy, and especially in the third trimester, both uterine blood flow and prostacyclin production by ovine uterine arteries are dramatically increased. We sought to determine if this is due, in part, to an increase in cyclooxygenase (COX) expression in the uterine artery endothelium. In this study we compared COX expression in uterine artery endothelium from nonpregnant and third-trimester pregnant (110-142 days' gestation) ewes. COX-2 expression was not detectable by Western blotting in uterine artery endothelium or vascular smooth muscle (VSM). In contrast, COX-1 expression was clearly observed in uterine artery. Immunohistochemical localization of COX-1 was endothelium > VSM, with both cell types showing an increase in COX-1 during the third trimester of pregnancy. COX-1 protein and messenger RNA (mRNA) levels were also detectable in collagenase dispersed endothelial cells, with expression of COX-1 in uterine artery endothelial cells dramatically increased during the third trimester of pregnancy at both the level of protein (346.4 +/- 28% of nonpregnant controls, P < 0.0005) and mRNA (51.04 +/- 7.98-fold of nonpregnant controls, P < 0.001). We conclude that the pregnancy-induced increases in prostacyclin pr...Continue Reading

References

Jun 1, 1978·The Journal of Clinical Endocrinology and Metabolism·R B EverettN F Gant
Oct 15, 1978·American Journal of Obstetrics and Gynecology·M K McLaughlinR A Chez
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W L XieD L Simmons
May 8, 1991·Biochimica Et Biophysica Acta·D L DeWitt
Jan 1, 1990·Prostaglandins·R R MagnessC R Rosenfeld
Nov 15, 1989·Biochemical and Biophysical Research Communications·G D RosenM J Holtzman
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M Fagan, A L Goldberg
Jan 1, 1988·The Journal of Clinical Endocrinology and Metabolism·G E HofmannT A Siddiqi
Dec 1, 1985·The Journal of Clinical Investigation·R R MagnessC R Rosenfeld
Aug 1, 1981·The Journal of Clinical Investigation·R P Naden, C R Rosenfeld
Sep 1, 1994·American Journal of Obstetrics and Gynecology·R R MagnessN F Gant
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L MasferrerK Seibert
Jul 25, 1996·Biochemical and Biophysical Research Communications·S N AlshihabiJ M Tarbell
Dec 1, 1996·Prostaglandins·A Arslan, H H Zingg
Jun 15, 1997·Analytical Biochemistry·D S Millican, I M Bird

❮ Previous
Next ❯

Citations

Jun 29, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Delrae M EckmanLorna G Moore
May 24, 2008·Reproductive Sciences·Katherine Chang, Lubo Zhang
Aug 16, 2011·Journal of Proteomics·Jayanth Ramadoss, Ronald R Magness
May 3, 2011·American Journal of Obstetrics and Gynecology·Joris van DrongelenMarc E A Spaanderman
Dec 1, 2000·Human Reproduction·J J Dalle LuccaN L Alsip
Apr 25, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P D Hurn, I M Macrae
Oct 25, 2017·Hypertension·Styliani Goulopoulou
Oct 13, 2001·Circulation Research·S T Davidge
Mar 1, 2005·Human Reproduction·C HermenegildoA Cano
Feb 12, 2019·Annual Review of Physiology·George OsolMaurizio Mandalà
Jun 3, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Cleofina Bosco, Chosica Buffet
Jan 17, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ian M BirdRonald R Magness
Sep 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C R Rosenfeld
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R R MagnessI M Bird
Mar 26, 2009·Reproductive Sciences·Cresta Wedel JonesGeorge Osol
Oct 16, 2004·The Journal of Biological Chemistry·Huimiao JiangThomas M McIntyre
Jul 27, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Oluwatobiloba OsikoyaStyliani Goulopoulou
Aug 28, 2021·International Journal of Molecular Sciences·Xiangqun Hu, Lubo Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.