Pregnancy rates in heifers and cows with cryopreserved sexed sperm: effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting

Theriogenology
J L SchenkG E Seidel

Abstract

Field trials were conducted to increase fertility with AI of flow-sorted, sexed bovine sperm. In the first trial, a novel competitive fertilization approach was used to compare pressures (30psi vs 50psi) for sorting sperm. Both X- and Y-sperm were sorted to approximately 95% purity at 30 and at 50psi; X-50+Y-30 (and the converse) were mixed in equal numbers for AI of heifers. Fetal sex divulged which treatment produced the pregnancy; 82% of pregnancies resulted from the 30psi treatment (P<0.05). Based on a similar approach, a new-pulsed laser did not damage sperm any more than the previous standard continuous wave laser. In a large field trial, sorting sperm at 40psi increased pregnancy rates in heifers relative to 50psi (42.3% vs 34.1%, n=367/group, P<0.05). Storing sperm for 20h before sorting at 40psi decreased pregnancy rates from 42.3% (n=367) to 36.8% (n=368; P<0.05). Breeding heifers with sexed sperm 55-56h after CIDR removal and PGF(2alpha) resulted in 34% (n=32) pregnant, compared to 49% (n=35) with fixed-time insemination 67-68h after CIDR removal (P>0.1). Lactating dairy cows pre-screened for normal reproductive tracts when OvSynch injections (GnRH, prostaglandin, GnRH) were initiated, had similar (P>0.1) pregnancy r...Continue Reading

References

Aug 1, 1995·Biology of Reproduction·D L Garner, L A Johnson
Mar 29, 2000·Theriogenology·J L SchenkG E Seidel
Mar 29, 2000·Theriogenology·G E SeidelD G Cran
Jan 23, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·George E Seidel, Duane L Garner
Mar 24, 2004·Animal Reproduction Science·F E O GarcíaT E M T Sánchez
Jul 24, 2004·Animal Reproduction Science·W M C MaxwellJ K O'Brien
Aug 30, 2005·Theriogenology·T K SuhG E Seidel
May 12, 2007·Society of Reproduction and Fertility Supplement·J L Schenk, G E Seidel

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Citations

Apr 1, 2014·Animal : an International Journal of Animal Bioscience·S T ButlerL Shalloo
Apr 1, 2014·Animal : an International Journal of Animal Bioscience·G E Seidel
Jun 22, 2012·Animal : an International Journal of Animal Bioscience·S KhalajzadehH Mehrbani Yeganeh
Nov 5, 2013·The Veterinary Clinics of North America. Food Animal Practice·George E Seidel
Dec 14, 2011·Animal Reproduction Science·José Henrique E DominguezFabio José C Faria
Nov 4, 2008·Theriogenology·S P de GraafW M C Maxwell
Oct 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·M Barceló-FimbresG E Seidel
Jan 29, 2014·Reproduction in Domestic Animals = Zuchthygiene·E KarakayaA Gumen
Aug 13, 2013·Journal of Dairy Science·Katelyn McCullockWilliam Wailes
Oct 21, 2016·The Journal of Reproduction and Development·Yasushi InabaTamás Somfai
Apr 4, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Phirawit ChuawongboonThevin Vongpralub
Feb 20, 2018·Journal of Dairy Science·D J CottleA G Fahey
Jul 17, 2018·Reproduction in Domestic Animals = Zuchthygiene·Ali JabarzarehVictor Cabrera
Jun 4, 2010·The Journal of Reproduction and Development·Yutaka FukuiHiroyuki Watanabe
Apr 8, 2020·Journal of Dairy Science·Ricardo C Chebel, Thiago Cunha
Aug 4, 2020·Reproduction in Domestic Animals = Zuchthygiene·Baris GunerAhmet Gumen
Nov 6, 2012·The Journal of Reproduction and Development·George E Seidel
Jan 1, 2019·Reproduction, Fertility, and Development·M MikkolaJ Taponen
Jan 19, 2019·Reproductive Medicine and Biology·Yousuke NaniwaKyoko Uchiyama
Mar 4, 2020·Tropical Animal Health and Production·Yulin HeTaiyong Yu

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