Preheating induced homogeneity of the small heat shock protein from Methanococcus jannaschii

Biochimica Et Biophysica Acta
Aoneng CaoLuhua Lai

Abstract

Small heat shock proteins usually exhibit increased chaperone-like activity either at high temperatures or after preheating. However, the activation mechanism is still unclear. In the current study, we investigated the preheating-activation process of Mj HSP16.5, using various biophysical methods. Although Mj HSP16.5 was reported to be the most monodispersed sHSPs, we found that the newly purified Mj HSP16.5 was actually heterogeneous. 85 degrees C-preheating could activate Mj HSP16.5 and turn it into a more compact homogeneous species at the same time. Different cooling rates after preheating did not change the activity of Mj HSP16.5, suggesting that the 85 degrees C-preheated Mj HSP16.5 is in the most active and also the most stable state. These results demonstrate that the activation process of Mj HSP16.5 might accompany a refolding process.

References

Sep 20, 1997·The Journal of Biological Chemistry·B Raman, C M Rao
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·R KimS H Kim
Aug 26, 1998·Nature·K K KimS H Kim
Feb 18, 1999·Current Biology : CB·P van den IJsselR A Quinlan
Dec 3, 1999·The EMBO Journal·M HaslbeckJ Buchner
Feb 19, 2000·Biochemical and Biophysical Research Communications·M R BurgioJ F Koretz
Apr 15, 2000·Journal of Molecular Biology·D A HaleyP L Stewart
Jun 23, 2000·Nature·E Nisbet
Nov 10, 2001·Nature Structural Biology·R L van MontfortE Vierling
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Jacob LebowitzPeter Schuck
Sep 26, 2002·The Journal of Biological Chemistry·Kim C Giese, Elizabeth Vierling
Dec 12, 2002·Cellular and Molecular Life Sciences : CMLS·M Haslbeck
Mar 15, 2003·The Journal of Biological Chemistry·Thusnelda StromerJohannes Buchner
Jun 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rosalind KimSung-Hou Kim
Jul 25, 2003·Biochemical and Biophysical Research Communications·Dong Ryoung KimKyeong Kyu Kim
Oct 3, 2003·Biochemical and Biophysical Research Communications·Xinmiao FuZengyi Chang
Jun 22, 2005·Journal of Molecular Biology·Titus M FranzmannJohannes Buchner
Oct 6, 2005·Nature Structural & Molecular Biology·Martin HaslbeckJohannes Buchner

❮ Previous
Next ❯

Citations

Oct 8, 2011·The Protein Journal·S Shirin ShahangianBijan Ranjbar
Nov 22, 2011·Biochemical and Biophysical Research Communications·Dong XiLuhua Lai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.