Preimplantation genetic diagnosis for chromosome rearrangements - one blastomere biopsy versus two blastomere biopsy.

Journal of Assisted Reproduction and Genetics
D BrodieT Osianlis

Abstract

Preimplantation Genetic Diagnosis (PGD) has proven to be a useful reproductive option for carriers of some chromosome rearrangements. The data presented in this study compares the impact of one versus two blastomere biopsy on the likelihood of achieving a PGD result, as well as the effect on subsequent embryo development and clinical outcomes. IVF-PGD couples had either one or two blastomeres biopsied from all embryos with ≥7 blastomeres on day 3 post oocyte collection. These blastomeres were assessed for the specific chromosome rearrangement using Fluorescent In-situ Hybridisation (FISH). Further embryo development was monitored on days 4 and 5. Clinical outcomes were assessed retrospectively. The data shows that statistically more embryos achieved a PGD result following two blastomere biopsy, compared with one blastomere biopsy (92 % versus 88 %, respectively). Furthermore it was found that embryo development and clinical outcomes were similar between the two biopsy groups. Based on this analysis it appears that the biopsy of two blastomeres from embryos with ≥7 blastomeres on day 3 is a valid and successful approach for couples presenting for IVF-PGD for a chromosome rearrangement.

References

Apr 24, 2003·Reproductive Biomedicine Online·Esther VelillaSantiago Munné
Jun 16, 2006·Human Reproduction·An MichielsCatherine Staessen
Feb 7, 2008·Reproductive Biomedicine Online·UNKNOWN Preimplantation Genetic Diagnosis International Society (PGDIS)
Oct 23, 2010·Human Reproduction·G L HartonUNKNOWN European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium/Embryology Special Interest Group

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Citations

Apr 6, 2013·Stem Cells and Development·Masood BazrgarHossein Baharvand
Sep 6, 2014·PloS One·Elena E ZakharovaAlexander S Krivokharchenko
Dec 19, 2015·Systems Biology in Reproductive Medicine·Tahereh HaghpanahHadi Azimi
Mar 5, 2016·BioMed Research International·Danilo CimadomoLaura Rienzi
Jul 16, 2014·Taiwanese Journal of Obstetrics & Gynecology·Shao-Ping Fred WengTsung-Chieh Jackson Wu
Oct 19, 2017·Expert Review of Molecular Diagnostics·Anver Kuliev, Svetlana Rechitsky
Jul 3, 2018·Panminerva Medica·Valeria RomanelliAntonio Capalbo
Jan 4, 2018·Systems Biology in Reproductive Medicine·Iman HalvaeiMohammad Ali Khalili
Dec 28, 2019·Obstetrical & Gynecological Survey·James M KemperAlon J Talmor
Aug 2, 2017·Journal of Assisted Reproduction and Genetics·Claire E Beyer, E Willats

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