Preimplantation mouse embryos express a heat-stable alkaline phosphatase

Biology of Reproduction
M L Lepire, C A Ziomek

Abstract

The cell surface alkaline phosphatase (APase) of the preimplantation mouse embryo has been characterized in situ by inhibition studies and fluorescent histochemistry. The embryonic APase has also been compared in the inhibition studies to the APase expressed on mouse F9 teratocarcinoma cells and, in some instances, to the APase of mouse intestinal epithelial cells. The embryonic APase was active over the pH range of 6.0-10.0, with the optimal pH for full activity in the range of 8.5-10.0. The embryonic APase was remarkably heat stable with significant loss of activity detected only after a 1 h incubation at 90 degrees C. A variety of specific and nonspecific APase inhibitors were applied to embryos to determine the nature of the APase isozymes expressed on these cells. The embryonic APase was totally resistant to levamisole, tetramisole, bromotetramisole, and L-homoarginine. The embryonic APase was inhibited by L-phenylalanine in a concentration-dependent fashion and by sodium arsenate, sodium vanadate, ethylenediamine tetraacetic acid, and slightly by 1,10-phenanthroline. The inhibition profile of the mouse embryonic APase, therefore, resembles most closely that reported for human placental APase with respect to heat stability...Continue Reading

Citations

Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S NarisawaJ L Millán
Oct 1, 1993·Molecular Reproduction and Development·N C TalbotA M Powell
Mar 15, 2015·Stem Cells International·Kateřina ŠtefkováJiří Pacherník
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K McDougallA Hahnel
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Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S NarisawaJ L Millán
Apr 23, 2020·Molecular Biology of the Cell·Ellen Youngsoo RimRoeland Nusse
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