Preimplantational embryo development and incidence of blastomere apoptosis in bovine somatic cell nuclear transfer embryos reconstructed with long-term cultured donor cells

Theriogenology
Goo JangWoo Suk Hwang

Abstract

This study was performed to investigate whether types and/or age of donor cells affect preimplantational embryo development and the incidence of apoptosis in bovine somatic cell nuclear transfer (SCNT) embryos. Bovine fetal or adult ear fibroblasts were isolated, cultured in vitro and categorized into fresh or long-term cultured cells in terms of population doublings (PD): in fetal fibroblasts, <16 being considered fresh and >50 being long-term cultured; in adult ear fibroblasts, <16 being considered fresh and >30 being long-term cultured. Bovine oocytes from slaughterhouse ovaries were matured in TCM-199, enucleated and reconstructed by SCNT. The reconstructed oocytes were fused, chemically activated, and cultured in modified synthetic oviduct fluid (mSOF) at 39 degrees C in a humidified atmosphere of 5% CO(2) air for 7 days. The early development of SCNT embryos was monitored under a microscope and the quality of blastocysts was assessed by differential counting of inner cell mass (ICM) and trophectoderm (TE) cells and by apoptosis detection in blastomeres using a terminal deoxynucleotidyl transferase-mediated d-UTP nick end-labeling (TUNEL) assay. As results, types and/or age of donor cells did not affect the rate of blastoc...Continue Reading

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Citations

Dec 21, 2007·The Journal of Reproduction and Development·Tatsuya FujimuraHiroshi Nagashima
Oct 10, 2009·The Journal of Reproduction and Development·Kanokwan SrirattanaRangsun Parnpai
Apr 6, 2006·Cell and Tissue Research·Guo Qing TongSoon Chye Ng
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Apr 11, 2006·Molecular Reproduction and Development·Jeffery M BetthauserGregory H Leno
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Jul 20, 2016·PloS One·Ji Hye LeeMin Kyu Kim
Dec 6, 2006·The Journal of Veterinary Medical Science·Byoung-Chol OhWoo-Suk Hwang

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