PMID: 3384154Jan 1, 1988Paper

Preliminary characterization and purification of in vitro encapsulation promoting factor: a peptide that mediates insect haemocyte adhesion

Developmental and Comparative Immunology
D H DaviesS B Vinson

Abstract

The granular cells and plasmatocytes (PLs) of Heliothis virescens form multicellular aggregations in vitro. This allows capsules to form around suitable targets. Prior trypsinization of the haemocytes abolishes their ability to encapsulate, and this function can be restored by adding plasma to the trypsinized cells. Trypsinized PLs were also unable to spread on a planar glass surface unless normal plasma was present. Plasma was subjected to a variety of treatments to determine the nature of the encapsulation promoting factor (EPF) using the in vitro encapsulation system as a bioassay. The data suggest EPF is a peptide; it is trypsin sensitive and moderately heat stable. Similar results were obtained when using spreading by trypsinized PLs as the bioassay. Dialysis using a 3,500 MW cut-off membrane also abolished encapsulation promoting activity. Protein-free extracts of plasma (crude EPF) has strong activity in both bioassays but does not agglutinate human erythrocytes. A single peak with strong activity in both bioassays was resolved after subjecting crude EPF to reversed-phase HPLC. This active material was purified after additional HPLC with a different solvent system.

References

Jan 1, 1987·Developmental and Comparative Immunology·D H Davies, T M Preston
Apr 1, 1974·The Australian Journal of Experimental Biology and Medical Science·C J Tyson, C R Jenkin
Jan 1, 1984·Developmental and Comparative Immunology·K SöderhällM Ramstedt

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Citations

Mar 27, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O SchmidtM Strand
Jul 1, 1994·Cell and Tissue Research·L L PechM R Strand
May 29, 2003·Journal of Insect Physiology·Robert J. ZakarianManfred E. Rau
May 29, 2003·Journal of Insect Physiology·M R. Strand, D Trudeau
Jun 1, 1997·Parasitology Today·Y Carton, A J Nappi
Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek

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