Preliminary characterization of extracellular allelochemicals of the toxic marine dinoflagellate Alexandrium tamarense using a Rhodomonas salina bioassay.

Marine Drugs
Haiyan MaA D Cembella

Abstract

Members of the marine dinoflagellate genus Alexandrium are known to exude allelochemicals, unrelated to well-known neurotoxins (PSP-toxins, spirolides), with negative effects on other phytoplankton and marine grazers. Physico/chemical characterization of extracellular lytic compounds of A. tamarense, quantified by Rhodomonas salina bioassay, showed that the lytic activity, and hence presumably the compounds were stable over wide ranges of temperatures and pH and were refractory to bacterial degradation. Two distinct lytic fractions were collected by reversed-phase solid-phase extraction. The more hydrophilic fraction accounted for about 2% of the whole lytic activity of the A. tamarense culture supernatant, while the less hydrophilic one accounted for about 98% of activity. Although temporal stability of the compounds is high, substantial losses were evident during purification. Lytic activity was best removed from aqueous phase with chloroform-methanol (3:1). A "pseudo-loss" of lytic activity in undisturbed and low-concentrated samples and high activity of an emulsion between aqueous and n-hexane phase after liquid-liquid partition are strong evidence for the presence of amphipathic compounds. Lytic activity in the early fract...Continue Reading

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Citations

Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Erik SelanderThomas Kiørboe
May 14, 2011·Marine Drugs·Olga MangoniAlfonso Mangoni
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kelsey L Poulson-EllestadBrook L Nunn
Jul 12, 2011·Toxicon : Official Journal of the International Society on Toxinology·Haiyan MaAllan Cembella
Jan 24, 2019·Environmental Microbiology Reports·Eva SörensonCatherine Legrand
Dec 9, 2010·Natural Product Reports·R Drew SiegJulia Kubanek
Mar 16, 2021·Ecotoxicology and Environmental Safety·Xiao-Tong MaoWei-Dong Yang
Mar 8, 2020·Developmental and Comparative Immunology·Malwenn LassudriePatricia Mirella da Silva

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Methods Mentioned

BETA
nuclear magnetic resonance

Software Mentioned

Statistica1

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