Preliminary characterization of the acetylcholine receptor in human erythrocytes

Journal of Supramolecular Structure
W H Huestis

Abstract

The response of human erythrocytes to cholinergic ligands was studied with an electron spin resonance assay. The membrane response to carbamyl choline was found to be antagonized by atropine and, in the absence of calcium, by tetrodotoxin. Experiments with resealed ghosts showed that the membrane response to carbamyl choline required ATP and calcium. Reductive alkylation of intact cells eliminated the cholinergic response, but the presence of saturating amounts of carbamyl choline protected the putative receptor against inactivation. Affinity labeling was used to demonstrate an apparent molecular weight of 41,000 for the carbamyl choline-binding species. A lipid vesicle extraction technique was used to induce a specific cation permeability defect in intact cells. Preliminary investigation of this phenomenon is described.

References

Apr 14, 1972·Biochemical and Biophysical Research Communications·H M McConnellB G McFarland
Jan 26, 1973·Biochimica Et Biophysica Acta·J R TrudellE N Cohen
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·T B StromJ P Merrill
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G K Humphries, H M McConnell
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P J DehlingerO H Griffith
Sep 23, 1974·Biochemical and Biophysical Research Communications·R J LefkowitzM G Caron
Nov 6, 1974·Biochemical and Biophysical Research Communications·L B DolapchievM Laskowski
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·V T MarchesiR E Scott
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A Karlin, D Cowburn
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A LevitzkiM L Steer
Jul 24, 1974·Biochemical and Biophysical Research Communications·V Sitaramam, T Ramasarma
Aug 4, 1971·Nature: New Biology·A C AllisonS De Petris
Sep 15, 1971·Nature: New Biology·J G Schofield
Jan 27, 1971·Journal of the American Chemical Society·W L Hubbell, H M McConnell
Jan 21, 1967·Nature·S B Carter
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

Citations

Apr 1, 1977·Life Sciences·R S AronstamM K MacNeil
Jan 1, 1982·Pharmacology & Therapeutics·A T EldefrawiM E Eldefrawi
Aug 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Joseph F HoffmanSulayman D Dib-Hajj
Jan 13, 1980·Brain Research·P D Wade, P S Timiras
Jan 1, 1993·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·S V Deshpande, R L Beissinger
May 1, 1979·Journal of Neurology, Neurosurgery, and Psychiatry·U Lindblom, R T Verrillo
Nov 1, 1977·CRC Critical Reviews in Biochemistry·H G Mautner

Related Concepts

Acetylcholine Sulfate (1: 1)
Adenosine Triphosphate, Chromium Ammonium Salt
Serum Proteins
Calcium
Carboptic
Plasma Membrane
Erythrocytes
Hemolysis
Hydrogen-Ion Concentration
Phosphatidylcholines

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