PMID: 45543Jan 1, 1978

Preliminary characterization of the delta-9 desaturase of Tetrahymena pyriformis W

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
H ShipiroJ L Rabinowitz

Abstract

1. In vitro assay conditions have been defined for measurement of delta 9 desaturase activity in Tetrahymena pyriformis W. 2. The reaction depends on the presence of oxygen and a reduced pyridine nucleotide cofactor. FAD supports a low level of enzymatic activity. 3. Both stearyl-CoA and palmityl-CoA are acceptable substrates. Oleate formation is maximal at 30 degrees C. 4. Delta-9 desaturase activity appears to be localized in the microsomal fraction. Delta-6 and/or delta 12 desaturase activities have also been observed. 5. When the specificity of the delta 9 desaturase towards stearyl-CoA and palmityl-CoA was observed at 30 and 16 degrees C it was found that lowering the assay temperature did not affect specificity. Stearyl-CoA was more readily desaturated at both temperatures. 6. Exogenous oleyl-CoA and diisopropylfluorophosphate had little effect on delta 9 desaturase activity. However, cyanide strongly inhibited desaturation and a sensitivity to sulfhydryl-binding reagents has also been demonstrated.

References

Mar 24, 1975·Biochimica Et Biophysica Acta·E L Pugh, M Kates
Feb 1, 1976·The Journal of protozoology·R L Conner, B Y Stewart
Apr 1, 1972·Archives of Biochemistry and Biophysics·N Oshino, R Sato
Feb 1, 1970·Analytical Biochemistry·F B MalloryR L Conner
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P StrittmatterR Redline
Oct 1, 1958·Biochimica Et Biophysica Acta·D K BLOOMFIELD, K BLOCH
Aug 1, 1963·Biochemical and Biophysical Research Communications·P W HOLLOWAYS J WAKIL
Nov 1, 1964·The Journal of protozoology·R L Conner, S G CLINE
Jul 2, 1962·Biochimica Et Biophysica Acta·J B MARSH, A T JAMES

Citations

Feb 1, 1981·The Journal of protozoology·J Bertram, J A Erwin

Related Concepts

Stearoyl-CoA Desaturase
Substrate Specificity
Tetrahymena pyriformis
Acyl CoA Desaturases

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