PMID: 7035940Dec 1, 1981Paper

Preliminary characterization of the major RNA species from Plasmodium falciparum

Molecular and Biochemical Parasitology
A C Vezza, W Trager

Abstract

Polyacrylamide gel electrophoresis of the total RNA extracted from cultures of Plasmodium falciparum resolved two major RNA components with estimated molecular weights of 1.3 X 10(6) and 0.72 - 0.74 X 10(6). Oligo(dT)-cellulose column chromatography of the total cellular RNA indicates that more than 90% of the total radiolabeled RNA lacks substantial poly(A) sequences (poly(A)-). Resolution of the poly(A)- fraction on polyacrylamide or agarose gels indicates that the majority of the poly(A)- RNAs are the 1.3 X 10(6) and 0.72 - 0.74 X 10(6) species. This observation was confirmed by two-dimensional oligonucleotide fingerprint analysis of the total cellular RNA as well as the poly(A)- RNA components. Oligonucleotide fingerprint analysis confirmed that the small RNA species is not a breakdown or cleavage product of the larger RNA component. Base ratio analysis of the large and small RNA species indicate that they are typically protozoan type with a low guanine + cytosine (G + C) content. The findings that these two RNA species (i) are the major cellular RNA species, (ii) lack substantial poly(A) sequences, (iii) have estimated molecular weights similar to the ribosomal RNAs obtained from other protozoa, (iv) have a low G + C conte...Continue Reading

References

Jan 1, 1975·Journal of Virology·E Hefti, D H Bishop
May 1, 1977·Journal of Virology·J ClewleyD H Bishop
Dec 1, 1978·Archives of Biochemistry and Biophysics·R T GarvinM M Weber
Aug 20, 1976·Science·W Trager, J B Jensen
Nov 1, 1975·The Journal of protozoology·I W ShermanJ Williamson
May 1, 1977·The Journal of protozoology·I W Sherman, L A Jones
Mar 1, 1976·Journal of General Microbiology·R Spencer, G A Cross
Dec 29, 1972·Journal of Molecular Evolution·P A Lava-SanchezA L Posta
Aug 1, 1970·Chemico-biological Interactions·D C Warhurst, J Williamson
Jan 1, 1970·Annual Review of Biochemistry·G Attardi, F Amaldi
Aug 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R T CookM J Fournier
Aug 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Aikawa, R T Cook
Sep 1, 1972·Analytical Biochemistry·R de Wachter, W Fiers
Jun 28, 1967·Journal of Molecular Biology·D H BishopS Spiegelman

❮ Previous
Next ❯

Citations

Jan 1, 1985·Transactions of the Royal Society of Tropical Medicine and Hygiene·M O de Rojas, M Wasserman
Feb 1, 1983·Molecular and Biochemical Parasitology·J F da Silveira, O Mercereau-Puijalon
Jul 1, 1983·Molecular and Biochemical Parasitology·J B Dame, T F McCutchan
Jul 1, 1984·Molecular and Biochemical Parasitology·F W Miller, J Ilan
Jan 15, 1987·Molecular and Biochemical Parasitology·D Shippen-LentzA C Vezza
Jan 15, 1988·Molecular and Biochemical Parasitology·D E Shippen-Lentz, A C Vezza
Jun 1, 1995·Molecular and Biochemical Parasitology·B KappesR M Franklin
Jul 15, 1992·European Journal of Biochemistry·B W SuetterlinR M Franklin
Nov 1, 1989·Molecular Microbiology·C J DelvesJ G Scaife
Oct 1, 1987·Annals of Tropical Medicine and Parasitology·W Trager
Dec 1, 1984·Journal of Bacteriology·C A Gritzmacher, R T Reese

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.