Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Nicola G A AbresciaD I Stuart

Abstract

PM2 (Corticoviridae) is a dsDNA bacteriophage which contains a lipid membrane beneath its icosahedral capsid. In this respect it resembles bacteriophage PRD1 (Tectiviridae), although it is not known whether the similarity extends to the detailed molecular architecture of the virus, for instance the fold of the major coat protein P2. Structural analysis of PM2 has been initiated and virus-derived P2 has been crystallized by sitting-nanodrop vapour diffusion. Crystals of P2 have been obtained in space group P2(1)2(1)2, with two trimers in the asymmetric unit and unit-cell parameters a = 171.1, b = 78.7, c = 130.1 A. The crystals diffract to 4 A resolution at the ESRF BM14 beamline (Grenoble, France) and the orientation of the non-crystallographic threefold axes, the spatial relationship between the two trimers and the packing of the trimers within the unit cell have been determined. The trimers form tightly packed layers consistent with the crystal morphology, possibly recapitulating aspects of the arrangement of subunits in the virus.

References

Feb 17, 1971·Nature: New Biology·S C HarrisonR M Franklin
Apr 1, 1968·Virology·R T Espejo, E S Canelo
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jul 23, 2002·Journal of Virology·Hanna M KiveläDennis H Bamford
Nov 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Narayanasamy NandhagopalMichael G Rossmann
Jun 12, 2003·Research in Microbiology·Dennis H Bamford
Aug 3, 2004·Nature Structural & Molecular Biology·Juha T HuiskonenSarah J Butcher
Nov 5, 2004·Nature·Nicola G A AbresciaJaana K H Bamford
Nov 5, 2004·Nature·Joseph J B CockburnDavid I Stuart
Dec 3, 2004·Molecular Cell·Stacy D BensonRoger M Burnett
Feb 9, 2005·Structure·Christopher J MayoRobert M Esnouf

❮ Previous
Next ❯

Citations

Nov 6, 2013·Acta Crystallographica. Section D, Biological Crystallography·David I Stuart, Nicola G A Abrescia
Dec 18, 2007·Journal of Bacteriology·Hanna M KiveläJaana K H Bamford
Mar 28, 2007·Current Opinion in Structural Biology·Juha T Huiskonen, Sarah J Butcher
Mar 12, 2013·Applied and Environmental Microbiology·Jumpei UchiyamaShigenobu Matsuzaki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.