Preliminary Evaluation of Glyceric Acid-producing Ability of Acidomonas methanolica NBRC104435 from Glycerol Containing Methanol

Journal of Oleo Science
Shun SatoHiroshi Habe

Abstract

Some acetic acid bacteria produce large amounts of glyceric acid (GA) from glycerol in culture broth. However, methanol, which is a major contaminant of raw glycerol derived from the biodiesel fuel industry, sharply decreases cell growth and GA production [AMB Express, 3, 20, 2013]. Thus, we evaluated the methylotrophic acetic acid bacterium Acidomonas methanolica NBRC104435 for its ability to produce GA from glycerol containing methanol. This strain accumulated GA in its culture broth when 1-3 wt% glycerol was available as a carbon source. We observed improved cell growth and GA accumulation when 1 vol% methanol was added to the 3-5 wt% glycerol medium. The maximum concentration of GA was 12.8 g/L in medium containing 3 wt% glycerol plus 1 vol% methanol. In addition, the enantiomeric excess (ee) of the GA produced was revealed to be 44%, indicating that this strain converted glycerol to d-GA with a lower enantioselectivity than other acetic acid bacteria, which had 70-99% ee.

References

Feb 19, 2002·Bioresource Technology·Seraphim Papanikolaou, George Aggelis
Oct 26, 2005·Journal of Bioscience and Bioengineering·Takeshi ItoNaomichi Nishio
Oct 15, 2008·Applied Microbiology and Biotechnology·Hiroshi HabeKeiji Sakaki
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·Hiroshi HabeKeiji Sakaki
Oct 20, 2009·Applied and Environmental Microbiology·Hiroshi HabeKeiji Sakaki
Feb 27, 2010·The Journal of Organic Chemistry·Annalisa GuaragnaGiovanni Palumbo
Dec 4, 2014·Journal of Bioscience and Bioengineering·Shun SatoHiroshi Habe

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