Preliminary investigation of human serum albumin-Vβ inhibition on toxic shock syndrome induced by staphylococcus enterotoxin B in vitro and in vivo

Toxicon : Official Journal of the International Society on Toxinology
Qifeng YuanYongqiang Jiang

Abstract

Staphylococcus enterotoxin B (SEB) is a superantigen that can induce massive activation of T cells with specific Vβ and inflammatory cytokine cascades, which mediate shock. To date, no SEB vaccine has been developed for preventing toxic shock syndrome (TSS). Here, we evaluated the therapeutic effect of a fusion protein human serum albumin-Vβ (HSA-Vβ) on TSS induced by SEB. Compared with Vβ, the preparation of HSA-Vβ was much easier to handle owing to its solubility. Affinity testing showed that HSA-Vβ had high affinity for SEB. In vitro results showed that HSA-Vβ could effectively inhibit interferon (IFN)-γ and tumor necrosis factor (TNF)-α secretion by human peripheral blood mononuclear cells. Moreover, in vivo, HSA-Vβ reduced IFN-γ and TNF-α levels in the serum and protected mice from SEB lethal challenge when administered simultaneously with SEB or 30 min after SEB. In summary, we simplified the preparation of Vβ by fusion with HSA, creating the HSA-Vβ protein, which effectively inhibited cytokine production and protected mice from lethal challenge with SEB. These data indicated that HSA-Vβ may represent a novel therapeutic strategy for the treatment of SEB-induced TSS.

References

Mar 1, 1979·Critical Care Medicine·M H WeilV K Puri
Dec 2, 1999·Immunologic Research·T Krakauer
May 16, 2002·Infection and Immunity·Martha L HaleBradley G Stiles
May 23, 2007·Nature Medicine·Rebecca A BuonpaneDavid M Kranz
Oct 4, 2007·Cancer Biology & Therapy·Ke-Ge JieYong-Qiang Jiang
May 28, 2010·Cancer Investigation·Hai-Rong WangYong-Qiang Jiang
Jan 31, 2013·Intensive Care Medicine·R P DellingerUNKNOWN Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup
Dec 12, 2013·BMJ : British Medical Journal·Smita PadhiUNKNOWN National Institute for Health and Care Excellence (NICE) Guideline Development Group
Mar 19, 2014·The New England Journal of Medicine·Pietro CaironiUNKNOWN ALBIOS Study Investigators

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